中文 
Novoprotein/近岸蛋白
Novoprotein/近岸蛋白
NovoRec? plus One step PCR Cloning Kit-100次
基本售價(jià)：2000.00元
產(chǎn)品描述一步定向克隆試劑盒是一款簡單、快速、高效的多片段DNA定向克隆產(chǎn)品，可以不依賴于連接酶，不受載體和目的片段的酶切位點(diǎn)限制，直接用同源重組的方法將PCR產(chǎn)物定向克隆至任意載體的任意位點(diǎn)，完成目的片段與載體相連接的基因克隆技術(shù)。該技術(shù)操作簡單，PCR產(chǎn)物一步定向克隆，目的片段與載體無縫連接，反應(yīng)時(shí)間短至10分鐘，陽性率達(dá)到95%以上，讓您輕松完成實(shí)驗(yàn)。保存條件收到后請(qǐng)立即低速離心保存在-20℃；此Kit可在-20℃長期保存，避免反復(fù)凍融。
NovoRec? plus One step PCR Cloning Kit-20次
基本售價(jià)：600.00元
產(chǎn)品描述一步定向克隆試劑盒是一款簡單、快速、高效的多片段DNA定向克隆產(chǎn)品，可以不依賴于連接酶，不受載體和目的片段的酶切位點(diǎn)限制，直接用同源重組的方法將PCR產(chǎn)物定向克隆至任意載體的任意位點(diǎn)，完成目的片段與載體相連接的基因克隆技術(shù)。該技術(shù)操作簡單，PCR產(chǎn)物一步定向克隆，目的片段與載體無縫連接，反應(yīng)時(shí)間短至10分鐘，陽性率達(dá)到95%以上，讓您輕松完成實(shí)驗(yàn)。保存條件收到后請(qǐng)立即低速離心保存在-20℃；此Kit可在-20℃長期保存，避免反復(fù)凍融。
NovoScript?Plus All-in-one 1st Strand cDNA Synthesis SuperMix-100次（20μl體系）
基本售價(jià)：1080.00元
產(chǎn)品描述第一鏈cDNA合成預(yù)混液是以mRNA或者總RNA為模板，高效合成第一鏈cDNA。本產(chǎn)品含有反轉(zhuǎn)錄反應(yīng)所需的全部試劑（NovoScript? II Reverse Transcriptase，RNase Inhibitor，Oligo(dT)18，Random N6，dNTPs）。反應(yīng)時(shí)只需要加入RNA模板和水即可，操作簡單，降低操作過程中的污染。制品中含有去基因組成分gDNA Purge，以RNA為模板進(jìn)行cDNA第一鏈合成時(shí)，可以同步去除基因組DNA污染，反應(yīng)結(jié)束后，75℃加熱5分鐘，就可以失活反轉(zhuǎn)錄酶，RNA酶抑制劑。合成的第一鏈cDNA能直接用于PCR或熒光定量PCR的模板，第二鏈cDNA的合成或線性RNA擴(kuò)增，也可用于需要用帶有放射性或非放射性核苷酸標(biāo)記第一鏈cDNA的實(shí)驗(yàn)。產(chǎn)品特點(diǎn)1)本制品采用耐高溫逆轉(zhuǎn)錄酶，大幅提高熱穩(wěn)定性和cDNA合成效率；2)本制品中添加的NovoScript? II Reverse Transcriptase無RNase H活性，可避免第一鏈cDNA合成過程中，RNA/DNA雜交模板鏈中RNA降解，保證cDNA合成的質(zhì)量；3)本制品中Random N6和Oligo(dT)18引物比例經(jīng)過優(yōu)化，可均勻合成樣品中的cDNA；4)本制品中含有去基因組成分，逆轉(zhuǎn)錄時(shí)可同步去除基因組DNA污染。保存條件-20℃。注意事項(xiàng)1)用DEPC處理實(shí)驗(yàn)用到的所有實(shí)驗(yàn)器材，或者購買已經(jīng)證明無核酸酶的實(shí)驗(yàn)用具，實(shí)驗(yàn)過程戴手套并經(jīng)常更換手套，避免RNA酶的污染。2)確保所用試劑中無RNA酶污染。3)試劑盒要嚴(yán)格密封保存。在逆轉(zhuǎn)錄過程中，所有管子要確?？蹏?yán)。4)純化過的RNA必須保證不含有鹽，金屬離子，乙醇和苯酚，以上成分會(huì)干擾第一鏈cDNA的合成反應(yīng)?？刹捎靡掖汲恋鞷NA的方法去除掉痕量污染物。5)為保證逆轉(zhuǎn)錄反應(yīng)的有效進(jìn)行，需使用高質(zhì)量的RNA模板。6)當(dāng)模板含量較低或后續(xù)PCR擴(kuò)增片段過長時(shí)可以適當(dāng)延長逆轉(zhuǎn)錄時(shí)間。7)2×NovoScript? Plus 1st Strand cDNA Synthesis SuperMix、gDNA Purge及2×NovoScript? Plus No RT Control含有甘油，使用前請(qǐng)充分混勻后并短暫離心收集至管底再進(jìn)行使用。僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
NovoScript?Plus All-in-one 1st Strand cDNA Synthesis SuperMix-50次（20μl體系）
基本售價(jià)：600.00元
產(chǎn)品描述第一鏈cDNA合成預(yù)混液是以mRNA或者總RNA為模板，高效合成第一鏈cDNA。本產(chǎn)品含有反轉(zhuǎn)錄反應(yīng)所需的全部試劑（NovoScript? II Reverse Transcriptase，RNase Inhibitor，Oligo(dT)18，Random N6，dNTPs）。反應(yīng)時(shí)只需要加入RNA模板和水即可，操作簡單，降低操作過程中的污染。制品中含有去基因組成分gDNA Purge，以RNA為模板進(jìn)行cDNA第一鏈合成時(shí)，可以同步去除基因組DNA污染，反應(yīng)結(jié)束后，75℃加熱5分鐘，就可以失活反轉(zhuǎn)錄酶，RNA酶抑制劑。合成的第一鏈cDNA能直接用于PCR或熒光定量PCR的模板，第二鏈cDNA的合成或線性RNA擴(kuò)增，也可用于需要用帶有放射性或非放射性核苷酸標(biāo)記第一鏈cDNA的實(shí)驗(yàn)。產(chǎn)品特點(diǎn)1)本制品采用耐高溫逆轉(zhuǎn)錄酶，大幅提高熱穩(wěn)定性和cDNA合成效率；2)本制品中添加的NovoScript? II Reverse Transcriptase無RNase H活性，可避免第一鏈cDNA合成過程中，RNA/DNA雜交模板鏈中RNA降解，保證cDNA合成的質(zhì)量；3)本制品中Random N6和Oligo(dT)18引物比例經(jīng)過優(yōu)化，可均勻合成樣品中的cDNA；4)本制品中含有去基因組成分，逆轉(zhuǎn)錄時(shí)可同步去除基因組DNA污染。保存條件-20℃。注意事項(xiàng)1)用DEPC處理實(shí)驗(yàn)用到的所有實(shí)驗(yàn)器材，或者購買已經(jīng)證明無核酸酶的實(shí)驗(yàn)用具，實(shí)驗(yàn)過程戴手套并經(jīng)常更換手套，避免RNA酶的污染。2)確保所用試劑中無RNA酶污染。3)試劑盒要嚴(yán)格密封保存。在逆轉(zhuǎn)錄過程中，所有管子要確?？蹏?yán)。4)純化過的RNA必須保證不含有鹽，金屬離子，乙醇和苯酚，以上成分會(huì)干擾第一鏈cDNA的合成反應(yīng)?？刹捎靡掖汲恋鞷NA的方法去除掉痕量污染物。5)為保證逆轉(zhuǎn)錄反應(yīng)的有效進(jìn)行，需使用高質(zhì)量的RNA模板。6)當(dāng)模板含量較低或后續(xù)PCR擴(kuò)增片段過長時(shí)可以適當(dāng)延長逆轉(zhuǎn)錄時(shí)間。7)2×NovoScript? Plus 1st Strand cDNA Synthesis SuperMix、gDNA Purge及2×NovoScript? Plus No RT Control含有甘油，使用前請(qǐng)充分混勻后并短暫離心收集至管底再進(jìn)行使用。僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
NovoStart? SYBR High-Sensitivity qPCR SuperMix-1ml×5
基本售價(jià)：1280.00元
產(chǎn)品描述本制品是采用SYBR Green I嵌合熒光法進(jìn)行Real Time PCR的專用試劑。預(yù)混液中已經(jīng)將DNA聚合酶、精心優(yōu)化的反應(yīng)Buffer、dNTPs、SYBR Green I等試劑預(yù)混在一起，是一種2×濃度的預(yù)混型試劑，進(jìn)行實(shí)驗(yàn)時(shí)，PCR反應(yīng)液的配制十分簡單方便。本制品使用新型抗體修飾的HotStart Taq DNA聚合酶，并結(jié)合精心優(yōu)化的反應(yīng)Buffer，具有更高的擴(kuò)增靈敏度，反應(yīng)特異性和擴(kuò)增效率，能夠在更寬廣的范圍內(nèi)進(jìn)行準(zhǔn)確定量。產(chǎn)品特點(diǎn)高靈敏度：Novoprotein精心配制的RealTime PCR專用2×SuperMix，具有更高的擴(kuò)增靈敏度和擴(kuò)增效率。高特異性：采用新型工藝制備抗體修飾的HotStart Taq DNA聚合酶，無需熱啟動(dòng)即可進(jìn)行PCR反應(yīng)，大大提高PCR擴(kuò)增的特異性?？旖荩篜CR反應(yīng)所必需試劑集于一管之中，數(shù)分鐘即可完成反應(yīng)體系配制。保存條件-20℃避光儲(chǔ)存。如需在一段時(shí)間內(nèi)經(jīng)常取用，可在2~8℃條件下儲(chǔ)存3個(gè)月。避免反復(fù)多次凍融。質(zhì)量控制純度檢測(cè)：所有組分經(jīng)檢測(cè)均無核酸內(nèi)切酶殘留、核酸外切酶殘留、核酸殘留。使用建議使用：請(qǐng)上下顛倒輕輕混合均勻，避免起泡，并經(jīng)輕微離心后使用。反應(yīng)液的配制、分裝請(qǐng)使用新的（無污染的）槍頭、Microtube等，避免污染。建議反應(yīng)體積為20～50μl。引物設(shè)計(jì)：一般為了增加靈敏度及擴(kuò)增效率且抑制非特異性反應(yīng)，擴(kuò)增目標(biāo)序列越短越好。建議設(shè)計(jì)成200bp以下。僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
NovoStart? SYBR High-Sensitivity qPCR SuperMix-1ml×25
基本售價(jià)：5670.00元
產(chǎn)品描述本制品是采用SYBR Green I嵌合熒光法進(jìn)行Real Time PCR的專用試劑。預(yù)混液中已經(jīng)將DNA聚合酶、精心優(yōu)化的反應(yīng)Buffer、dNTPs、SYBR Green I等試劑預(yù)混在一起，是一種2×濃度的預(yù)混型試劑，進(jìn)行實(shí)驗(yàn)時(shí)，PCR反應(yīng)液的配制十分簡單方便。本制品使用新型抗體修飾的HotStart Taq DNA聚合酶，并結(jié)合精心優(yōu)化的反應(yīng)Buffer，具有更高的擴(kuò)增靈敏度，反應(yīng)特異性和擴(kuò)增效率，能夠在更寬廣的范圍內(nèi)進(jìn)行準(zhǔn)確定量。產(chǎn)品特點(diǎn)高靈敏度：Novoprotein精心配制的RealTime PCR專用2×SuperMix，具有更高的擴(kuò)增靈敏度和擴(kuò)增效率。高特異性：采用新型工藝制備抗體修飾的HotStart Taq DNA聚合酶，無需熱啟動(dòng)即可進(jìn)行PCR反應(yīng)，大大提高PCR擴(kuò)增的特異性?？旖荩篜CR反應(yīng)所必需試劑集于一管之中，數(shù)分鐘即可完成反應(yīng)體系配制。保存條件-20℃避光儲(chǔ)存。如需在一段時(shí)間內(nèi)經(jīng)常取用，可在2~8℃條件下儲(chǔ)存3個(gè)月。避免反復(fù)多次凍融。質(zhì)量控制純度檢測(cè)：所有組分經(jīng)檢測(cè)均無核酸內(nèi)切酶殘留、核酸外切酶殘留、核酸殘留。使用建議使用：請(qǐng)上下顛倒輕輕混合均勻，避免起泡，并經(jīng)輕微離心后使用。反應(yīng)液的配制、分裝請(qǐng)使用新的（無污染的）槍頭、Microtube等，避免污染。建議反應(yīng)體積為20～50μl。引物設(shè)計(jì)：一般為了增加靈敏度及擴(kuò)增效率且抑制非特異性反應(yīng)，擴(kuò)增目標(biāo)序列越短越好。建議設(shè)計(jì)成200bp以下。僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
NovoStart?SYBR qPCR SuperMix Plus-1ml×25
基本售價(jià)：5670.00元
產(chǎn)品描述本制品是采用SYBR Green I嵌合熒光法進(jìn)行Real Time PCR的專用試劑。預(yù)混液中已經(jīng)將DNA聚合酶、精心優(yōu)化的反應(yīng)Buffer、dNTPs、SYBR Green I等試劑預(yù)混在一起，是一種2×濃度的預(yù)混型試劑，進(jìn)行實(shí)驗(yàn)時(shí)，PCR反應(yīng)液的配制十分簡單方便。本制品使用新型抗體修飾的HotStart Taq DNA聚合酶，并結(jié)合精心優(yōu)化的反應(yīng)Buffer，從而有效抑制低溫下的非特異性擴(kuò)增，提高反應(yīng)特異性和擴(kuò)增效率，能夠在更寬廣的范圍內(nèi)進(jìn)行準(zhǔn)確定量。產(chǎn)品特點(diǎn)高特異性：采用新型工藝制備抗體修飾的HotStart Taq DNA聚合酶，無需熱啟動(dòng)即可進(jìn)行PCR反應(yīng)，大大提高PCR擴(kuò)增的特異性。高效：Novoprotein精心配制的RealTime PCR專用2×SuperMix，具有更高的擴(kuò)增效率和擴(kuò)增靈敏度?？旖荩篜CR反應(yīng)所必需試劑集于一管之中，數(shù)分鐘即可完成反應(yīng)體系配制。保存條件-20℃避光儲(chǔ)存。如需在一段時(shí)間內(nèi)經(jīng)常取用，可在2~8℃條件下儲(chǔ)存3個(gè)月。避免反復(fù)多次凍融。質(zhì)量控制純度檢測(cè)：所有組分經(jīng)檢測(cè)均無核酸內(nèi)切酶殘留、核酸外切酶殘留、核酸殘留。使用建議使用：請(qǐng)上下顛倒輕輕混合均勻，避免起泡，并經(jīng)輕微離心后使用。反應(yīng)液的配制、分裝請(qǐng)使用新的（無污染的）槍頭、Microtube等，避免污染。建議反應(yīng)體積為20～50μl。引物設(shè)計(jì)：一般為了增加靈敏度及擴(kuò)增效率且抑制非特異性反應(yīng)，擴(kuò)增目標(biāo)序列越短越好。建議設(shè)計(jì)成200bp以下。僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
NovoStart? Fast SYBR qPCR SuperMix-1ml×25
基本售價(jià)：6336.00元
產(chǎn)品描述本制品是采用 SYBR Green I 嵌合熒光法進(jìn)行快速 Real Time PCR 的 2×濃度預(yù)混型試劑，PCR 反應(yīng)液的配制十分簡單方便。本制品使用突變型 Taq DNA 聚合酶，結(jié)合精心優(yōu)化的反應(yīng) Buffer，可有效抑制低溫下的非特異性擴(kuò)增，實(shí)現(xiàn)快速 PCR 反應(yīng)，并且能夠在寬廣的范圍內(nèi)對(duì)靶基因進(jìn)行準(zhǔn)確、快速、可重復(fù)的定量檢測(cè)。產(chǎn)品特點(diǎn)快速：實(shí)現(xiàn)快速熒光定量，相較于傳統(tǒng) qPCR，反應(yīng)時(shí)間減少 50%左右；保存條件-20℃避光儲(chǔ)存，避免反復(fù)凍融。使用建議使用：請(qǐng)上下顛倒輕輕混合均勻，避免起泡，并經(jīng)輕微離心后使用。反應(yīng)液的配制、分裝請(qǐng)使用新的（無污染的）槍頭、Microtube等，避免污染。建議反應(yīng)體積為20～50μl。僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。Novoprotein是蘇州近岸蛋白質(zhì)科技股份有限公司的注冊(cè)商標(biāo)。請(qǐng)?jiān)L問我們的注冊(cè)商標(biāo)頁面。其他商標(biāo)是其他所有者的財(cái)產(chǎn)。
NovoStart? Fast SYBR qPCR SuperMix-Imlx5
基本售價(jià)：1408.00元
產(chǎn)品描述本制品是采用 SYBR Green I 嵌合熒光法進(jìn)行快速 Real Time PCR 的 2×濃度預(yù)混型試劑，PCR 反應(yīng)液的配制十分簡單方便。本制品使用突變型 Taq DNA 聚合酶，結(jié)合精心優(yōu)化的反應(yīng) Buffer，可有效抑制低溫下的非特異性擴(kuò)增，實(shí)現(xiàn)快速 PCR 反應(yīng)，并且能夠在寬廣的范圍內(nèi)對(duì)靶基因進(jìn)行準(zhǔn)確、快速、可重復(fù)的定量檢測(cè)。產(chǎn)品特點(diǎn)快速：實(shí)現(xiàn)快速熒光定量，相較于傳統(tǒng) qPCR，反應(yīng)時(shí)間減少 50%左右；保存條件-20℃避光儲(chǔ)存，避免反復(fù)凍融。使用建議使用：請(qǐng)上下顛倒輕輕混合均勻，避免起泡，并經(jīng)輕微離心后使用。反應(yīng)液的配制、分裝請(qǐng)使用新的（無污染的）槍頭、Microtube等，避免污染。建議反應(yīng)體積為20～50μl。僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。Novoprotein是蘇州近岸蛋白質(zhì)科技股份有限公司的注冊(cè)商標(biāo)。請(qǐng)?jiān)L問我們的注冊(cè)商標(biāo)頁面。其他商標(biāo)是其他所有者的財(cái)產(chǎn)。
NovoNGS? CUT&Tag? 4.0 High-Sensitivity Kit (for Illumina?)-24次
基本售價(jià)：17400.00元
產(chǎn)品描述Cleavage Under Target & Tagmentation (CUT&Tag?)是一種新興的DNA-蛋白質(zhì)互作研究方法。CUT&Tag? 以融合了protein A/G 的Tn5 轉(zhuǎn)座酶為核心，融合蛋白通過protein A/G 與抗體結(jié)合，使得Tn5 被栓系在靶位點(diǎn)周圍，從而在目的位點(diǎn)附近進(jìn)行酶切反應(yīng)，并同時(shí)引入二代測(cè)序所必需的接頭序列，產(chǎn)物DNA 經(jīng)過后續(xù)提取與PCR 擴(kuò)增后，得到直接用于測(cè)序的文庫。CUT&Tag?與傳統(tǒng)的ChIP-Seq 技術(shù)相比較，實(shí)驗(yàn)不需要超聲打斷，也不需要傳統(tǒng)的連接法添加測(cè)序接頭，操作簡單、周期短、信噪比高、重復(fù)性好、細(xì)胞用量少，為極低起始細(xì)胞量和單細(xì)胞測(cè)序研究提供了可能性。自 2019 年 CUT&Tag 技術(shù)誕生以來，經(jīng)過大量的實(shí)踐，發(fā)現(xiàn)該技術(shù)在組蛋白修飾及部分轉(zhuǎn)錄因子與 DNA 互作研究中可以獲得高信噪比的結(jié)果，但是針對(duì)一些與 DNA 結(jié)合力較弱或者結(jié)合具有高度動(dòng)態(tài)性的 DNA 結(jié)合蛋白，較難得到理想的實(shí)驗(yàn)結(jié)果。針對(duì)此問題，近岸蛋白開發(fā)了可對(duì)細(xì)胞進(jìn)行高濃度甲醛交聯(lián)的CUT&Tag實(shí)驗(yàn)流程。為轉(zhuǎn)錄因子 -DNA 互作研究提供一種新的思路，這種需要甲醛交聯(lián)的CUT&Tag實(shí)驗(yàn)被稱為fcCUT&Tag（formaldehyde crosslinking CUT&Tag）。NovoNGS? CUT&Tag? 4.0 High-Sensitivity Kit (for Illumina）試劑盒包含常規(guī)非交聯(lián)細(xì)胞CUT&Tag及甲醛交聯(lián)細(xì)胞fcCUT&Tag實(shí)驗(yàn)中所需的各種緩沖液、ConA磁珠、DNA純化磁珠、引物等全套建庫試劑，可以用于組蛋白修飾和轉(zhuǎn)錄因子的染色質(zhì)結(jié)合狀態(tài)研究。與傳統(tǒng)的CUT&Tag試劑盒相比，該試劑盒既可以對(duì)自然狀態(tài)的細(xì)胞進(jìn)行蛋白質(zhì)-DNA互作研究，也可以對(duì)高濃度甲醛交聯(lián)的細(xì)胞進(jìn)行蛋白質(zhì)-DNA互作研究，一盒兩用。試劑盒包含CUT&Tag? 實(shí)驗(yàn)中孵育抗體、轉(zhuǎn)座體、構(gòu)建文庫的相關(guān)試劑，可以用于組蛋白修飾和轉(zhuǎn)錄因子的染色質(zhì)結(jié)合狀態(tài)研究。本試劑盒包含高活性的ChiTag? pAG-Tn5, 相較于最初版本的pA-Tn5 轉(zhuǎn)座酶，pAG結(jié)構(gòu)域?qū)ν谩⑹蟮榷喾N來源抗體具有廣譜的強(qiáng)親和性，在實(shí)驗(yàn)抗體的選擇上具有更大的空間。針對(duì)CUT&Tag?打斷產(chǎn)物DNA 提取過程中小片段丟失的問題，本試劑盒提供優(yōu)化的Tagment DNA Extract Beads, 相較于常規(guī)的片段分選磁珠可以完整的回收CUT&Tag? 產(chǎn)物中的小片段DNA, 大大簡化了酚氯仿-乙醇沉淀法的繁瑣操作。此外，本試劑盒還提供了陽性抗體H3K4me3（兔抗）以及相應(yīng)的二抗（羊抗兔IgG) ，用于陽性對(duì)照實(shí)驗(yàn)（僅B 包裝提供陽性抗體）。圖1： DNA-Target Protein-Antibody-ChiTag?結(jié)合示意圖 圖2： CUT&Tag 實(shí)驗(yàn)流程簡圖 產(chǎn)品用途DNA－蛋白質(zhì)互作研究。在常見的哺乳動(dòng)物細(xì)胞上用于替代傳統(tǒng)的ChIP-Seq 技術(shù)，特別適用于細(xì)胞數(shù)量較少的樣本，也可應(yīng)用于經(jīng)過處理的植物和酵母樣本上。保存條件詳見說明書僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
NovoNGS? CUT&Tag? 4.0 High-Sensitivity Kit (for Illumina?)-12次
基本售價(jià)：9000.00元
產(chǎn)品描述Cleavage Under Target & Tagmentation (CUT&Tag?)是一種新興的DNA-蛋白質(zhì)互作研究方法。CUT&Tag? 以融合了protein A/G 的Tn5 轉(zhuǎn)座酶為核心，融合蛋白通過protein A/G 與抗體結(jié)合，使得Tn5 被栓系在靶位點(diǎn)周圍，從而在目的位點(diǎn)附近進(jìn)行酶切反應(yīng)，并同時(shí)引入二代測(cè)序所必需的接頭序列，產(chǎn)物DNA 經(jīng)過后續(xù)提取與PCR 擴(kuò)增后，得到直接用于測(cè)序的文庫。CUT&Tag?與傳統(tǒng)的ChIP-Seq 技術(shù)相比較，實(shí)驗(yàn)不需要超聲打斷，也不需要傳統(tǒng)的連接法添加測(cè)序接頭，操作簡單、周期短、信噪比高、重復(fù)性好、細(xì)胞用量少，為極低起始細(xì)胞量和單細(xì)胞測(cè)序研究提供了可能性。自 2019 年 CUT&Tag 技術(shù)誕生以來，經(jīng)過大量的實(shí)踐，發(fā)現(xiàn)該技術(shù)在組蛋白修飾及部分轉(zhuǎn)錄因子與 DNA 互作研究中可以獲得高信噪比的結(jié)果，但是針對(duì)一些與 DNA 結(jié)合力較弱或者結(jié)合具有高度動(dòng)態(tài)性的 DNA 結(jié)合蛋白，較難得到理想的實(shí)驗(yàn)結(jié)果。針對(duì)此問題，近岸蛋白開發(fā)了可對(duì)細(xì)胞進(jìn)行高濃度甲醛交聯(lián)的CUT&Tag實(shí)驗(yàn)流程。為轉(zhuǎn)錄因子 -DNA 互作研究提供一種新的思路，這種需要甲醛交聯(lián)的CUT&Tag實(shí)驗(yàn)被稱為fcCUT&Tag（formaldehyde crosslinking CUT&Tag）。NovoNGS? CUT&Tag? 4.0 High-Sensitivity Kit (for Illumina）試劑盒包含常規(guī)非交聯(lián)細(xì)胞CUT&Tag及甲醛交聯(lián)細(xì)胞fcCUT&Tag實(shí)驗(yàn)中所需的各種緩沖液、ConA磁珠、DNA純化磁珠、引物等全套建庫試劑，可以用于組蛋白修飾和轉(zhuǎn)錄因子的染色質(zhì)結(jié)合狀態(tài)研究。與傳統(tǒng)的CUT&Tag試劑盒相比，該試劑盒既可以對(duì)自然狀態(tài)的細(xì)胞進(jìn)行蛋白質(zhì)-DNA互作研究，也可以對(duì)高濃度甲醛交聯(lián)的細(xì)胞進(jìn)行蛋白質(zhì)-DNA互作研究，一盒兩用。試劑盒包含CUT&Tag? 實(shí)驗(yàn)中孵育抗體、轉(zhuǎn)座體、構(gòu)建文庫的相關(guān)試劑，可以用于組蛋白修飾和轉(zhuǎn)錄因子的染色質(zhì)結(jié)合狀態(tài)研究。本試劑盒包含高活性的ChiTag? pAG-Tn5, 相較于最初版本的pA-Tn5 轉(zhuǎn)座酶，pAG結(jié)構(gòu)域?qū)ν?、鼠等多種來源抗體具有廣譜的強(qiáng)親和性，在實(shí)驗(yàn)抗體的選擇上具有更大的空間。針對(duì)CUT&Tag?打斷產(chǎn)物DNA 提取過程中小片段丟失的問題，本試劑盒提供優(yōu)化的Tagment DNA Extract Beads, 相較于常規(guī)的片段分選磁珠可以完整的回收CUT&Tag? 產(chǎn)物中的小片段DNA, 大大簡化了酚氯仿-乙醇沉淀法的繁瑣操作。此外，本試劑盒還提供了陽性抗體H3K4me3（兔抗）以及相應(yīng)的二抗（羊抗兔IgG) ，用于陽性對(duì)照實(shí)驗(yàn)（僅B 包裝提供陽性抗體）。圖1： DNA-Target Protein-Antibody-ChiTag?結(jié)合示意圖圖2： CUT&Tag 實(shí)驗(yàn)流程簡圖產(chǎn)品用途DNA－蛋白質(zhì)互作研究。在常見的哺乳動(dòng)物細(xì)胞上用于替代傳統(tǒng)的ChIP-Seq 技術(shù)，特別適用于細(xì)胞數(shù)量較少的樣本，也可應(yīng)用于經(jīng)過處理的植物和酵母樣本上。保存條件詳見說明書僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。Novoprotein是蘇州近岸蛋白質(zhì)科技股份有限公司的注冊(cè)商標(biāo)。請(qǐng)?jiān)L問我們的注冊(cè)商標(biāo)頁面。其他商標(biāo)是其他所有者的財(cái)產(chǎn)。
Recombinant Human SHH 細(xì)胞因子
基本售價(jià)：840.00元
Recombinant Human SHH 細(xì)胞因子 產(chǎn)品描述Recombinant Human Sonic Hedgehog is produced by our E.coli expression system and the target gene encoding Cys24-Gly197 is expressed.蛋白編號(hào)Q15465 產(chǎn)品別稱Sonic Hedgehog Protein; SHH; HHG-1分子量19.69 KDa表觀分子量19 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, 1mM DTT, pH 7.5.內(nèi)毒素Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test.純度-SDS-PAGEGreater than 95% as determined by reducing SDS-PAGE. (QC verified) 復(fù)溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運(yùn)輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產(chǎn)品背景Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases. Human SHH is expressed as a 45kDa precursor, and undergoes a series of processing during secretion. After the removal of the signal peptide, a protease within the C-terminal domain catalyzes the cleavage of SHH into a 20 kDa N-terminal signaling domain (SHH-N) and a 25 kDa C-terminal domain (SHH-C). SHH-N has the “all signaling” capability. SHH-N binds to the 12 pass transmembrane protein Patched (Ptc) on cell surface, which releases the repression of the activity of Smoothened (Smo), a G-protein coupled receptor, by Ptc.僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。Novoprotein是蘇州近岸蛋白質(zhì)科技股份有限公司的注冊(cè)商標(biāo)。請(qǐng)?jiān)L問我們的注冊(cè)商標(biāo)頁面。其他商標(biāo)是其他所有者的財(cái)產(chǎn)。
Recombinant Human FGF-10(Cat. No.:CR11)
基本售價(jià)：14400.00元
Recombinant Human FGF-10(Cat. No.:CR11) 該產(chǎn)品共有2篇文獻(xiàn)引用 產(chǎn)品描述Recombinant Human Fibroblast Growth Factor 10 is produced by our E.coli expression system and the target gene encoding Gln38-Ser208 is expressed.蛋白編號(hào)O15520 產(chǎn)品別稱Fibroblast growth factor 10;FGF-10;Keratinocyte growth factor 2;FGF10;KGF-2;KGF2分子量19.5 KDa表觀分子量19-22 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 8.0.內(nèi)毒素Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test.純度-SDS-PAGEGreater than 95% as determined by reducing SDS-PAGE. 純度-SEC-HPLCGreater than 95% as determined by SEC-HPLC. (Regularly tested) 生物活性-Cell Based AssayMouse Gastric organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) , R-spondin 1 (Cat#CX83) and FGF-10 (Cat#CR11). The organoids showed good morphology. Mouse bile duct organoids were cultured with EGF (Cat#C029) , R-spondin 1 (Cat#CX83) ,FGF-10 (Cat#CR11) and HGF (Cat#CJ72). The organoids showed good morphology. 復(fù)溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運(yùn)輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產(chǎn)品背景Fibroblast growth factor 10 (FGF-10, KGF-2), is a member of the fibroblast growth factor (FGF) family that includes FGF-3, -7, and -22. KGF-2 is secreted by mesenchymal cells and associates with extracellular FGF-BP. It preferentially binds and activates epithelial cell FGFR2 and interacts more weakly with FGFR1. It plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. It exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 is required for normal branching morphogenesis. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
Recombinant Human RSPO1 (C-6His)(Cat. No.:CX83)
基本售價(jià)：17600.00元
Recombinant Human RSPO1 (C-6His)(Cat. No.:CX83) 該產(chǎn)品共有5篇文獻(xiàn)引用 產(chǎn)品描述Recombinant Human R-spondin-1 is produced by our Mammalian expression system and the target gene encoding Ser21-Ala263 is expressed with a 6His tag at the C-terminus.蛋白編號(hào)Q2MKA7 產(chǎn)品別稱RSPO1; R-spondin1; RP11-566C13.1; CRISTIN3; FLJ40906; RSPO Rspo1; R-spondin; Rspondin; RP23-325M14.2; Roof plate-specific spondin-1分子量27.8 KDa表觀分子量40 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.內(nèi)毒素Less than 0.01 EU/μg as determined by LAL test.純度-SDS-PAGEGreater than 95% as determined by reducing SDS-PAGE. 生物活性-Cell Based AssayMeasured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 4.06 ng/ml. Mouse intestinal organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) and R-spondin 1 (Cat#CX83). After five days of culture, the organoids showed good morphology and germination rate. Mouse Colon organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) , and R-spondin 1 (Cat#CX83). The organoids showed good morphology. Mouse Gastric organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) , R-spondin 1 (Cat#CX83) and FGF-10 (Cat#CR11). The organoids showed good morphology. Mouse bile duct organoids were cultured with EGF (Cat#C029) , R-spondin 1 (Cat#CX83) ,FGF-10 (Cat#CR11) and HGF (Cat#CJ72). The organoids showed good morphology. Human intestinal organoids (ipsc source) were cultured with Activin A(Cat#C687) , BMP-4(sample) , EGF (Cat#C029) , Wnt3a (Cat#C22R), Noggin (Cat#C028), R-spondin 1 (Cat#CX83) and FGF-4 (Cat#CR08) . The organoids showed good morphology. Human endometrial epithelial organoids were cultured with EGF (Cat#CH28) and R-spondin 1 (Cat#CX83). The organoids showed good morphology. Data were kindly provided by Novoprotein's customer. Thanks for her contribution. 復(fù)溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運(yùn)輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產(chǎn)品背景RSPO1 is a secreted protein,containing 2 FU(furin-like) repeats and 1 TSP type-1 domain and belonging to the R-spondin family. RSPO1 is required for the early development of gonads, regardless of sex. It has been found in mice only eleven days after fertilization. To induce cell proliferation, it acts synergistically with WNT4. They help stabilize β catenin, which activates downstream targets. RSPO1 is necessary in female sex development. It augments the WNT/β catenin pathway to oppose male sex development. In critical gonadal stages, between six and nine weeks after fertilization, the ovaries upregulate it while the testes downregulate it. RSPO1 can potentially aid in the treatment of mucositis, which is characterized by inflammation of the oral cavity. This unfortunate condition often accompanies chemotherapy and radiation in cancer patients with head and neck tumors.僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
Recombinant Human KGF(Cat. No.:CH73)
基本售價(jià)：19200.00元
Recombinant Human KGF(Cat. No.:CH73) 該產(chǎn)品共有1篇文獻(xiàn)引用 產(chǎn)品描述Recombinant Human Fibroblast Growth Factor 7/Keratinocyte Growth Factor is produced by our E.coli expression system and the target gene encoding Cys32-Thr194 is expressed.蛋白編號(hào)P21781 產(chǎn)品別稱Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF分子量18.9 KDa表觀分子量17 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of 20mM Tris，1mM EDTA，5% Trehalose, 0.02% Tween 80, pH 8.0.內(nèi)毒素Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.純度-SDS-PAGEGreater than 95% as determined by reducing SDS-PAGE. (QC verified) 純度-SEC-HPLCGreater than 95% as determined by SEC-HPLC. (Regularly tested) 復(fù)溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運(yùn)輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產(chǎn)品背景Fibroblast growth factor 7 (FGF7) is a secreted protein which is mainly located in epithelial cells and belongs to the heparin-binding growth factors family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. It is possible major paracrine effector of normal epithelial cell proliferation.僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
Recombinant Human PECAM-1 (C-6His)(Cat. No.:CS74)
基本售價(jià)：400.00元
Recombinant Human PECAM-1 (C-6His)(Cat. No.:CS74) 產(chǎn)品描述Recombinant Human Platelet Endothelial Cell Adhesion Molecule is produced by our Mammalian expression system and the target gene encoding Gln28-Lys601 is expressed with a 6His tag at the C-terminus.蛋白編號(hào)AAH51822.1 產(chǎn)品別稱Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA; PECA1; CD31; PECAM1分子量65.3 KDa表觀分子量75-110 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of PBS, 1mM EDTA, pH 7.4.內(nèi)毒素 Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.復(fù)溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運(yùn)輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產(chǎn)品背景Platelet Endothelial Cell Adhesion Molecule (PECAM1, CD31), is a heavily glycosylated transmembrane protein belonging to the immunoglobulin (Ig) superfamily of cell adhesion molecules. CD31 is composed of an extracellular domain (ECD) of 574 amino acids (aa) containing six Ig-like domains, a transmembrane domain, and a 118 aa cytoplasmic domain. CD31 is highly expressed on endothelial cells and at a lower level on platelets, granulocytes, macrophages, dendritic cells, T and B cells, and natural killer (NK) cells. It is involved in cell adhesion and is required for transepithelial migration of leukocytes (TEM). CD31 acts as a homophilic receptor through its extracellular domain and is involved in downstream signaling via its cytoplasmic domain. This domain contains highly conserved ITIM motifs which, once tyrosine phosphorylated, recruit and activate the signaling molecules Src and SHP2. The resulting inhibition of TCR signaling increases the activation threshold of T cells, thus reinforcing peripheral tolerance and preventing development of autoimmunity. CD31 additionally regulates immune responses by acting as a key inhibitory receptor in dendritic cell development. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells.僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
Recombinant Mouse IL-4 (C-6His)(Cat. No.:CK74)
基本售價(jià)：17600.00元
Recombinant Mouse IL-4 (C-6His)(Cat. No.:CK74) 該產(chǎn)品共有24篇文獻(xiàn)引用 產(chǎn)品描述Recombinant Mouse Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His21-Ser140 is expressed with a 6His tag at the C-terminus.蛋白編號(hào)P07750 產(chǎn)品別稱Interleukin-4; IL-4; IL4; B-cell IgG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1; BSF-1; IGG1 induction factor; Lymphocyte stimulatory factor 1分子量14.6 KDa表觀分子量15-19 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.內(nèi)毒素 Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.復(fù)溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運(yùn)輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產(chǎn)品背景Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
NovoStart SYBR qPCR SuperMix Plus試劑
基本售價(jià)：1280.00元
NovoStart SYBR qPCR SuperMix Plus試劑 該產(chǎn)品共有552篇文獻(xiàn)引用 產(chǎn)品描述本制品是采用SYBR Green I嵌合熒光法進(jìn)行Real Time PCR的專用試劑。預(yù)混液中已經(jīng)將DNA聚合酶、精心優(yōu)化的反應(yīng)Buffer、dNTPs、SYBR Green I等試劑預(yù)混在一起，是一種2×濃度的預(yù)混型試劑，進(jìn)行實(shí)驗(yàn)時(shí)，PCR反應(yīng)液的配制十分簡單方便。本制品使用新型抗體修飾的HotStart Taq DNA聚合酶，并結(jié)合精心優(yōu)化的反應(yīng)Buffer，從而有效抑制低溫下的非特異性擴(kuò)增，提高反應(yīng)特異性和擴(kuò)增效率，能夠在更寬廣的范圍內(nèi)進(jìn)行準(zhǔn)確定量。產(chǎn)品特點(diǎn)高特異性：采用新型工藝制備抗體修飾的HotStart Taq DNA聚合酶，無需熱啟動(dòng)即可進(jìn)行PCR反應(yīng)，大大提高PCR擴(kuò)增的特異性。高效：Novoprotein精心配制的RealTime PCR專用2×SuperMix，具有更高的擴(kuò)增效率和擴(kuò)增靈敏度?？旖荩篜CR反應(yīng)所必需試劑集于一管之中，數(shù)分鐘即可完成反應(yīng)體系配制。保存條件-20℃避光儲(chǔ)存。如需在一段時(shí)間內(nèi)經(jīng)常取用，可在2~8℃條件下儲(chǔ)存3個(gè)月。避免反復(fù)多次凍融。質(zhì)量控制純度檢測(cè)：所有組分經(jīng)檢測(cè)均無核酸內(nèi)切酶殘留、核酸外切酶殘留、核酸殘留。使用建議使用：請(qǐng)上下顛倒輕輕混合均勻，避免起泡，并經(jīng)輕微離心后使用。反應(yīng)液的配制、分裝請(qǐng)使用新的（無污染的）槍頭、Microtube等，避免污染。建議反應(yīng)體積為20～50μl。引物設(shè)計(jì)：一般為了增加靈敏度及擴(kuò)增效率且抑制非特異性反應(yīng)，擴(kuò)增目標(biāo)序列越短越好。建議設(shè)計(jì)成200bp以下。僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
Recombinant Mouse TNF alpha
基本售價(jià)：2520.00元
Recombinant Mouse TNF alpha 產(chǎn)品描述Recombinant Mouse Tumor Necrosis Factor Alpha is produced by our E.coli expression system and the target gene encoding Asp89-Leu235 is expressed.蛋白編號(hào)P06804 產(chǎn)品別稱Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; Tumor Necrosis Factor; Membrane Form; Tumor Necrosis Factor; Soluble Form; Tnf; Tnfa; Tnfsf2分子量16.4 KDa表觀分子量14 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.內(nèi)毒素Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test.純度-SDS-PAGE
RNase R
基本售價(jià)：2000.00元
RNase R 產(chǎn)品描述核糖核酸酶R (Ribonuclease R，RNase R) 來源于大腸桿菌RNR超家族，是一種鎂離子依賴性的3'—5'核糖核酸外切酶，可從3'—5'方向?qū)NA逐步切割成二核苷酸和三核苷酸。RNase R幾乎能夠消化所有線性的RNA，但不易消化環(huán)狀RNA、套索結(jié)構(gòu)RNA和3'端突出少于7個(gè)核苷酸的短雙鏈RNA分子。 RNase R常用于基因表達(dá)和可變剪切研究，可消化線性RNA以富集環(huán)狀RNA或套索結(jié)構(gòu)RNA。保存條件-20±5℃僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
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