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Caspase 1活性檢測(cè)試劑盒(分光光度法)
基本售價(jià)：500.00元
Caspase 1 Activity Assay Kit(Colorimetric Method)中文名稱:Caspase 1活性檢測(cè)試劑盒(分光光度法)貨號(hào):E-CK-A381檢測(cè)原理 Caspase 1也稱interkeukin 1β conberting enzyme (ICE)，是caspase家族中唯一可以剪切IL-1β前體蛋白或IL-18前體產(chǎn)生相應(yīng)成熟的細(xì)胞因子的caspase。Caspase 11可以剪切45 kD的caspase 1前體蛋白，產(chǎn)生20 kD和10 kD的片段，這兩個(gè)片段可以形成異源二聚體 (heterodimer)，并進(jìn)一步由兩個(gè)異源二聚體形成四聚體。Caspase 1可以通過剪切Bcl-XL來調(diào)節(jié)細(xì)胞凋亡，并通過剪切細(xì)胞因子的前體來調(diào)節(jié)相關(guān)的免疫反應(yīng)，也可以通過水解Gastermin D (GSDMD)來調(diào)節(jié)細(xì)胞焦亡。本caspase 1活性檢測(cè)試劑盒是基于caspase 1可以催化底物Ac-YVAD-pNA產(chǎn)生黃色的pNA (p-nitroaniline)，pNA在405 nm附近有強(qiáng)吸收，從而可以通過測(cè)定吸光度來檢測(cè)caspase 1的活性。基本信息 應(yīng)用類型細(xì)胞凋亡-Caspase檢測(cè)類型定性分析或定量分析檢測(cè)方法比色法樣本類型細(xì)胞，組織檢測(cè)時(shí)間4-6 hours檢測(cè)儀器分光光度計(jì)自備試劑PBS(E-BC-R187)，Bradford蛋白測(cè)定試劑盒(E-BC-K168-S或E-BC-K168-M)保存溫度-20℃，避光運(yùn)輸條件冰袋保質(zhì)期6 months
人二胺氧化酶(DAO)酶聯(lián)免疫吸附測(cè)定試劑盒
基本售價(jià)：3200.00元
產(chǎn)品貨號(hào):E-EL-H1241c 中文名:人二胺氧化酶(DAO)酶聯(lián)免疫吸附測(cè)定試劑盒別稱:AOC1, ABP1, Amiloride Binding Protein 1, Amine Oxidase(Copper-Containing)用途 該試劑盒用于體外定量檢測(cè)Human 血清，血漿或其他生物體液中DAO濃度檢測(cè)原理 本試劑盒采用雙抗體夾心ELISA法。用抗人DAO抗體包被于酶標(biāo)板上，實(shí)驗(yàn)時(shí)樣品或標(biāo)準(zhǔn)品中的人DAO會(huì)與包被抗體結(jié)合，游離的成分被洗去。依次加入生物素化的抗人DAO抗體和辣根過氧化物酶標(biāo)記的親和素?？谷薉AO抗體與結(jié)合在包被抗體上的人DAO結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物，游離的成分被洗去。加入顯色底物(TMB)，TMB在辣根過氧化物酶的催化下呈現(xiàn)藍(lán)色，加終止液后變成黃色。用酶標(biāo)儀在450nm波長(zhǎng)處測(cè)OD值，DAO濃度與OD450值之間呈正比，通過繪制標(biāo)準(zhǔn)曲線計(jì)算出樣品中DAO的濃度。反應(yīng)類型 夾心法規(guī)格 96T反應(yīng)時(shí)間 3.5h反應(yīng)性 Human檢測(cè)方法 Colormetric檢測(cè)范圍 3.13—200 ng/mL靈敏度 1.88 ng/mL樣本體積 100μL樣本類型 血清，血漿或其他生物體液特異性 可檢測(cè)樣本中的人DAO，且與其它相關(guān)蛋白無明顯交叉反應(yīng)。重復(fù)性 板內(nèi)，板間變異系數(shù)均<10%。典型數(shù)據(jù) 由于實(shí)驗(yàn)操作條件的不同（如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等），標(biāo)準(zhǔn)曲線的OD值會(huì)有所差異。以下數(shù)據(jù)和曲線僅供參考，實(shí)驗(yàn)者需根據(jù)自己的實(shí)驗(yàn)建立標(biāo)準(zhǔn)曲線。(ng/mL) O.D Average Corrected 2002.4072.4412.4242.3351001.5471.6051.5761.487500.9180.8960.9070.817250.4660.4840.4750.38612.50.280.2780.2790.196.250.1980.1680.1830.0943.130.1290.1470.1380.04900.0870.090.089--精密度板內(nèi)精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測(cè)20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測(cè)20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(ng/mL) 10.7428.2690.3810.9527.5989.37Standarddeviation 0.681.293.070.641.433.93C V (%) 6.334.563.405.845.184.40回收率 分別往5個(gè)不同樣本中添加已知濃度的目標(biāo)蛋白，做回收實(shí)驗(yàn)，得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 92-10698EDTA plasma (n=8) 85-10092Cell culture media (n=8) 89-10295線性 分別往5個(gè)樣本中添加已知濃度的目標(biāo)蛋白，做回收實(shí)驗(yàn)，得出回收率范圍及平均回收率。將5個(gè)樣本分別稀釋2倍，4倍，8倍，16倍做回收實(shí)驗(yàn)，得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)94-10789-10195-109Average (%)99941011:4Range (%)87-10385-10088-103Average (%)9491951:8Range (%)88-10586-10088-104Average (%)9692951:16Range (%)89-10182-9682-96Average (%)948888試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周；如果一周以后才使用試劑盒，請(qǐng)拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規(guī)格 保存條件 ELISA酶標(biāo)板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃，可存放6個(gè)月凍干標(biāo)準(zhǔn)品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結(jié)合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光)，可存放6個(gè)月標(biāo)準(zhǔn)品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個(gè)月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結(jié)合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應(yīng)終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產(chǎn)品說明書Product Description1 份質(zhì)檢報(bào)告Certificate of Analysis1 份說明：所有試劑瓶蓋須旋緊以防止蒸發(fā)和微生物的污染。試劑體積以實(shí)際發(fā)貨版說明書為準(zhǔn)。相關(guān)試劑在分裝時(shí)會(huì)比標(biāo)簽上標(biāo)明的體積稍多一些，請(qǐng)?jiān)谑褂脮r(shí)量取而非直接倒出。試驗(yàn)所需自備物品 1. 酶標(biāo)儀(450nm波長(zhǎng)濾光片)2. 高精度移液器，EP管及一次性吸頭：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱，雙蒸水或去離子水4. 吸水紙
即用型正常山羊血清
基本售價(jià)：40.00元
產(chǎn)品貨號(hào):E-IR-R110 中文名:即用型正常山羊血清別稱:山羊血清,Normal Goat,封閉液，IHC封閉液，IF封閉液產(chǎn)品簡(jiǎn)介 本品為正常山羊血清加入穩(wěn)定劑，作為封閉液可效減少IHC/IF實(shí)驗(yàn)的非特異染色從而降低背景。 產(chǎn)品組成 產(chǎn)品編號(hào) 產(chǎn)品名稱 5 mL 10 mL 50 mL Storage E-IR-R110 Normal Goat Blocking Buffer (Ready-to-Use) 5 mL 10 mL 50 mL 2~8°C 產(chǎn)品使用說明 本產(chǎn)品為即用型山羊血清，可直接使用。 作為對(duì)照IgG使用時(shí)，用量和其它IgG的用量應(yīng)相同。對(duì)照IgG用量過多可能會(huì)導(dǎo)致背景過高。 作為免疫組化封閉液，請(qǐng)直接滴加到組織切片上進(jìn)行封閉。 保存條件 2~8°C保存，有效期12個(gè)月。 注意事項(xiàng) 1.本產(chǎn)品僅限于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。2.為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
Recombinant Human FGF-10(Cat. No.:CR11)
基本售價(jià)：14400.00元
Recombinant Human FGF-10(Cat. No.:CR11) 該產(chǎn)品共有2篇文獻(xiàn)引用 產(chǎn)品描述Recombinant Human Fibroblast Growth Factor 10 is produced by our E.coli expression system and the target gene encoding Gln38-Ser208 is expressed.蛋白編號(hào)O15520 產(chǎn)品別稱Fibroblast growth factor 10;FGF-10;Keratinocyte growth factor 2;FGF10;KGF-2;KGF2分子量19.5 KDa表觀分子量19-22 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM NaCl, pH 8.0.內(nèi)毒素Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test.純度-SDS-PAGEGreater than 95% as determined by reducing SDS-PAGE. 純度-SEC-HPLCGreater than 95% as determined by SEC-HPLC. (Regularly tested) 生物活性-Cell Based AssayMouse Gastric organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) , R-spondin 1 (Cat#CX83) and FGF-10 (Cat#CR11). The organoids showed good morphology. Mouse bile duct organoids were cultured with EGF (Cat#C029) , R-spondin 1 (Cat#CX83) ,FGF-10 (Cat#CR11) and HGF (Cat#CJ72). The organoids showed good morphology. 復(fù)溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運(yùn)輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產(chǎn)品背景Fibroblast growth factor 10 (FGF-10, KGF-2), is a member of the fibroblast growth factor (FGF) family that includes FGF-3, -7, and -22. KGF-2 is secreted by mesenchymal cells and associates with extracellular FGF-BP. It preferentially binds and activates epithelial cell FGFR2 and interacts more weakly with FGFR1. It plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. It exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. FGF10 is required for normal branching morphogenesis. Defects in FGF10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands (ALSG). ALSG has variable expressivity, and affected individuals may have aplasia or hypoplasia of the lacrimal, parotid, submandibular and sublingual glands and absence of the lacrimal puncta. The disorder is characterized by irritable eyes, recurrent eye infections, epiphora (constant tearing) and xerostomia (dryness of the mouth), which increases the risk of dental erosion, dental caries, periodontal disease and oral infections.僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
Recombinant Human RSPO1 (C-6His)(Cat. No.:CX83)
基本售價(jià)：17600.00元
Recombinant Human RSPO1 (C-6His)(Cat. No.:CX83) 該產(chǎn)品共有5篇文獻(xiàn)引用 產(chǎn)品描述Recombinant Human R-spondin-1 is produced by our Mammalian expression system and the target gene encoding Ser21-Ala263 is expressed with a 6His tag at the C-terminus.蛋白編號(hào)Q2MKA7 產(chǎn)品別稱RSPO1; R-spondin1; RP11-566C13.1; CRISTIN3; FLJ40906; RSPO Rspo1; R-spondin; Rspondin; RP23-325M14.2; Roof plate-specific spondin-1分子量27.8 KDa表觀分子量40 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.內(nèi)毒素Less than 0.01 EU/μg as determined by LAL test.純度-SDS-PAGEGreater than 95% as determined by reducing SDS-PAGE. 生物活性-Cell Based AssayMeasured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 4.06 ng/ml. Mouse intestinal organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) and R-spondin 1 (Cat#CX83). After five days of culture, the organoids showed good morphology and germination rate. Mouse Colon organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) , and R-spondin 1 (Cat#CX83). The organoids showed good morphology. Mouse Gastric organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) , R-spondin 1 (Cat#CX83) and FGF-10 (Cat#CR11). The organoids showed good morphology. Mouse bile duct organoids were cultured with EGF (Cat#C029) , R-spondin 1 (Cat#CX83) ,FGF-10 (Cat#CR11) and HGF (Cat#CJ72). The organoids showed good morphology. Human intestinal organoids (ipsc source) were cultured with Activin A(Cat#C687) , BMP-4(sample) , EGF (Cat#C029) , Wnt3a (Cat#C22R), Noggin (Cat#C028), R-spondin 1 (Cat#CX83) and FGF-4 (Cat#CR08) . The organoids showed good morphology. Human endometrial epithelial organoids were cultured with EGF (Cat#CH28) and R-spondin 1 (Cat#CX83). The organoids showed good morphology. Data were kindly provided by Novoprotein's customer. Thanks for her contribution. 復(fù)溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運(yùn)輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產(chǎn)品背景RSPO1 is a secreted protein,containing 2 FU(furin-like) repeats and 1 TSP type-1 domain and belonging to the R-spondin family. RSPO1 is required for the early development of gonads, regardless of sex. It has been found in mice only eleven days after fertilization. To induce cell proliferation, it acts synergistically with WNT4. They help stabilize β catenin, which activates downstream targets. RSPO1 is necessary in female sex development. It augments the WNT/β catenin pathway to oppose male sex development. In critical gonadal stages, between six and nine weeks after fertilization, the ovaries upregulate it while the testes downregulate it. RSPO1 can potentially aid in the treatment of mucositis, which is characterized by inflammation of the oral cavity. This unfortunate condition often accompanies chemotherapy and radiation in cancer patients with head and neck tumors.僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
Recombinant Human KGF(Cat. No.:CH73)
基本售價(jià)：19200.00元
Recombinant Human KGF(Cat. No.:CH73) 該產(chǎn)品共有1篇文獻(xiàn)引用 產(chǎn)品描述Recombinant Human Fibroblast Growth Factor 7/Keratinocyte Growth Factor is produced by our E.coli expression system and the target gene encoding Cys32-Thr194 is expressed.蛋白編號(hào)P21781 產(chǎn)品別稱Fibroblast growth factor 7; FGF-7; Heparin-binding growth factor 7; HBGF-7; Keratinocyte growth factor; FGF7; KGF分子量18.9 KDa表觀分子量17 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of 20mM Tris，1mM EDTA，5% Trehalose, 0.02% Tween 80, pH 8.0.內(nèi)毒素Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.純度-SDS-PAGEGreater than 95% as determined by reducing SDS-PAGE. (QC verified) 純度-SEC-HPLCGreater than 95% as determined by SEC-HPLC. (Regularly tested) 復(fù)溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運(yùn)輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產(chǎn)品背景Fibroblast growth factor 7 (FGF7) is a secreted protein which is mainly located in epithelial cells and belongs to the heparin-binding growth factors family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. It is possible major paracrine effector of normal epithelial cell proliferation.僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
Recombinant Human PECAM-1 (C-6His)(Cat. No.:CS74)
基本售價(jià)：400.00元
Recombinant Human PECAM-1 (C-6His)(Cat. No.:CS74) 產(chǎn)品描述Recombinant Human Platelet Endothelial Cell Adhesion Molecule is produced by our Mammalian expression system and the target gene encoding Gln28-Lys601 is expressed with a 6His tag at the C-terminus.蛋白編號(hào)AAH51822.1 產(chǎn)品別稱Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA; PECA1; CD31; PECAM1分子量65.3 KDa表觀分子量75-110 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of PBS, 1mM EDTA, pH 7.4.內(nèi)毒素 Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.復(fù)溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運(yùn)輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產(chǎn)品背景Platelet Endothelial Cell Adhesion Molecule (PECAM1, CD31), is a heavily glycosylated transmembrane protein belonging to the immunoglobulin (Ig) superfamily of cell adhesion molecules. CD31 is composed of an extracellular domain (ECD) of 574 amino acids (aa) containing six Ig-like domains, a transmembrane domain, and a 118 aa cytoplasmic domain. CD31 is highly expressed on endothelial cells and at a lower level on platelets, granulocytes, macrophages, dendritic cells, T and B cells, and natural killer (NK) cells. It is involved in cell adhesion and is required for transepithelial migration of leukocytes (TEM). CD31 acts as a homophilic receptor through its extracellular domain and is involved in downstream signaling via its cytoplasmic domain. This domain contains highly conserved ITIM motifs which, once tyrosine phosphorylated, recruit and activate the signaling molecules Src and SHP2. The resulting inhibition of TCR signaling increases the activation threshold of T cells, thus reinforcing peripheral tolerance and preventing development of autoimmunity. CD31 additionally regulates immune responses by acting as a key inhibitory receptor in dendritic cell development. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells.僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
Recombinant Mouse IL-4 (C-6His)(Cat. No.:CK74)
基本售價(jià)：17600.00元
Recombinant Mouse IL-4 (C-6His)(Cat. No.:CK74) 該產(chǎn)品共有24篇文獻(xiàn)引用 產(chǎn)品描述Recombinant Mouse Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His21-Ser140 is expressed with a 6His tag at the C-terminus.蛋白編號(hào)P07750 產(chǎn)品別稱Interleukin-4; IL-4; IL4; B-cell IgG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1; BSF-1; IGG1 induction factor; Lymphocyte stimulatory factor 1分子量14.6 KDa表觀分子量15-19 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.內(nèi)毒素 Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.復(fù)溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 保存條件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.運(yùn)輸條件The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.產(chǎn)品背景Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
NovoStart SYBR qPCR SuperMix Plus試劑
基本售價(jià)：1280.00元
NovoStart SYBR qPCR SuperMix Plus試劑 該產(chǎn)品共有552篇文獻(xiàn)引用 產(chǎn)品描述本制品是采用SYBR Green I嵌合熒光法進(jìn)行Real Time PCR的專用試劑。預(yù)混液中已經(jīng)將DNA聚合酶、精心優(yōu)化的反應(yīng)Buffer、dNTPs、SYBR Green I等試劑預(yù)混在一起，是一種2×濃度的預(yù)混型試劑，進(jìn)行實(shí)驗(yàn)時(shí)，PCR反應(yīng)液的配制十分簡(jiǎn)單方便。本制品使用新型抗體修飾的HotStart Taq DNA聚合酶，并結(jié)合精心優(yōu)化的反應(yīng)Buffer，從而有效抑制低溫下的非特異性擴(kuò)增，提高反應(yīng)特異性和擴(kuò)增效率，能夠在更寬廣的范圍內(nèi)進(jìn)行準(zhǔn)確定量。產(chǎn)品特點(diǎn)高特異性：采用新型工藝制備抗體修飾的HotStart Taq DNA聚合酶，無需熱啟動(dòng)即可進(jìn)行PCR反應(yīng)，大大提高PCR擴(kuò)增的特異性。高效：Novoprotein精心配制的RealTime PCR專用2×SuperMix，具有更高的擴(kuò)增效率和擴(kuò)增靈敏度?？旖荩篜CR反應(yīng)所必需試劑集于一管之中，數(shù)分鐘即可完成反應(yīng)體系配制。保存條件-20℃避光儲(chǔ)存。如需在一段時(shí)間內(nèi)經(jīng)常取用，可在2~8℃條件下儲(chǔ)存3個(gè)月。避免反復(fù)多次凍融。質(zhì)量控制純度檢測(cè)：所有組分經(jīng)檢測(cè)均無核酸內(nèi)切酶殘留、核酸外切酶殘留、核酸殘留。使用建議使用：請(qǐng)上下顛倒輕輕混合均勻，避免起泡，并經(jīng)輕微離心后使用。反應(yīng)液的配制、分裝請(qǐng)使用新的（無污染的）槍頭、Microtube等，避免污染。建議反應(yīng)體積為20～50μl。引物設(shè)計(jì)：一般為了增加靈敏度及擴(kuò)增效率且抑制非特異性反應(yīng)，擴(kuò)增目標(biāo)序列越短越好。建議設(shè)計(jì)成200bp以下。僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
Recombinant Mouse TNF alpha
基本售價(jià)：2520.00元
Recombinant Mouse TNF alpha 產(chǎn)品描述Recombinant Mouse Tumor Necrosis Factor Alpha is produced by our E.coli expression system and the target gene encoding Asp89-Leu235 is expressed.蛋白編號(hào)P06804 產(chǎn)品別稱Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; Tumor Necrosis Factor; Membrane Form; Tumor Necrosis Factor; Soluble Form; Tnf; Tnfa; Tnfsf2分子量16.4 KDa表觀分子量14 KDa, reducing conditions劑型描述Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.內(nèi)毒素Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test.純度-SDS-PAGE
RNase R
基本售價(jià)：2000.00元
RNase R 產(chǎn)品描述核糖核酸酶R (Ribonuclease R，RNase R) 來源于大腸桿菌RNR超家族，是一種鎂離子依賴性的3'—5'核糖核酸外切酶，可從3'—5'方向?qū)NA逐步切割成二核苷酸和三核苷酸。RNase R幾乎能夠消化所有線性的RNA，但不易消化環(huán)狀RNA、套索結(jié)構(gòu)RNA和3'端突出少于7個(gè)核苷酸的短雙鏈RNA分子。 RNase R常用于基因表達(dá)和可變剪切研究，可消化線性RNA以富集環(huán)狀RNA或套索結(jié)構(gòu)RNA。保存條件-20±5℃僅供科研或生產(chǎn)使用，不可直接應(yīng)用于人體。
Lysis dilution buffer（150 mM NaCl/5 mM EDTA）500ml
基本售價(jià)：170.00元
Lysis dilution buffer（150 mM NaCl/5 mM EDTA）500ml
TripleSelect無動(dòng)物源常溫胰酶
基本售價(jià)：260.00元
TripleSelect無動(dòng)物源常溫胰酶 【保存條件】 室溫保存12個(gè)月, 2–8°C或–20°C保存24個(gè)月【概述】 TripleSelect與胰蛋白酶一樣，可裂解賴氨酸和精氨酸C-末端上的肽鍵。但是，TripleSelect無與倫比的純度提高了特異性，其原因在于只有一種酶發(fā)揮作用。這減少了胰蛋白酶和其他提取物中存在的多種酶的裂解作用造成的損害?！井a(chǎn)品特點(diǎn)】 對(duì)細(xì)胞作用溫和：純度提高了特異性，其原因在于只有一種酶發(fā)揮作用。這減少了胰蛋白酶和其他提取物中由于多種酶切割造成的損害。在室溫下保持穩(wěn)定：在室溫或4°C下可保持穩(wěn)定12-24個(gè)月，因此易于儲(chǔ)存和處理、方便快捷。無動(dòng)物來源蛋白：與常用豬胰蛋白酶不同，本試劑不含動(dòng)物來源成分。無需終止，易于使用：無需使用胰蛋白酶抑制劑（如FBS）終止消化，PBS或DMEM等稀釋后自動(dòng)失活?！臼褂媒ㄗh】 1.使用前將TripleSelect和完整的生長(zhǎng)培養(yǎng)基恢復(fù)至室溫。2.將待消化細(xì)胞培養(yǎng)器皿中培養(yǎng)去除。3.使用DPBS（無鈣鎂）清洗細(xì)胞，清洗完去除DPBS液體。4.加入相應(yīng)體積的TripleSelect消化液（如25cm2瓶中加入1-2ml TripleSelect消化液，75cm2瓶中加入5ml TripleSelect消化液）5.37°C孵育至細(xì)胞變圓脫落（鏡下觀察），可輕拍培養(yǎng)瓶/皿。6.加入5ml完全培養(yǎng)基至培養(yǎng)瓶/皿（體積根據(jù)不同面積培養(yǎng)瓶/皿），可輕輕吹打使細(xì)胞更好分離。7.將含細(xì)胞的液體移入15ml無菌離心管中。以200-500g轉(zhuǎn)速離心5-10分鐘使細(xì)胞沉淀。8.去除上清后，加入含血清的完全培養(yǎng)液使細(xì)胞沉淀重新懸浮，即可用于后續(xù)實(shí)驗(yàn)【注意事項(xiàng)】 1. 注意無菌操作，盡量避免污染。2. 為了您的安全和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
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