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伊文斯藍(lán)染色液(0.5%)
基本售價：150.00元
伊文斯藍(lán)染色液(0.5%)產(chǎn)品簡介CAS314-13-6英文名稱Evan's Blue Stain Solution, 0.5%別名；伊文思蘭；偶氮藍(lán)；依文氏蘭；依文思藍(lán)；依文斯蘭；埃文斯藍(lán)；6,6'-[[3,3'-二甲基(1,1'-二苯基)-4,4'-二基]雙(偶氮基)]雙(4-氨基-5-羥基-1,3-萘二磺酸)四鈉鹽分子式C34H24N6Na4O14S4分子量960.81儲存條件室溫,避光保存,有效期1年規(guī)格100mL 500mL單位瓶伊文斯蘭(Evans Blue)又稱偶氮藍(lán)。伊文斯蘭與臺盼藍(lán)都是細(xì)胞活性染料，常用于檢測細(xì)胞膜的完整性和細(xì)胞是否存活?；罴?xì)胞因有外排功能而無法被伊文斯蘭染成藍(lán)色，而死細(xì)胞會被染成淡藍(lán)色。因此可以通過此方法在顯微鏡下區(qū)分死細(xì)胞與活細(xì)胞，但無法區(qū)分死亡與壞死。備注：以上數(shù)據(jù)均來自公開文獻(xiàn), Solarbio暫未進(jìn)行獨立驗證, 僅供參考。These protocols are for reference only. Solarbio does not independently validate these methods.
巴氏吸管，5 mL，獨立包裝，滅菌 318516
基本售價：448.00元
巴氏吸管，5 mL，獨立包裝，滅菌 318516 產(chǎn)品詳情?管壁流動性好，易于控制?透明度好，有刻度線，易于觀察?韌性好，可以在一定角度下彎曲，適用進(jìn)入微型以及異形容器操作?彈性好，適用于快速轉(zhuǎn)移?使用方便，精密度好，滴量的重復(fù)性好?每個包裝都有獨立的貨號批號標(biāo)識，便于質(zhì)量追蹤和溯源?無熱原，無內(nèi)毒素，無細(xì)胞毒性，無溶血性?EO滅菌
赤霉素(GA3)
基本售價：80.00元
赤霉素(GA3)產(chǎn)品簡介英文名稱Gibberellic acidCAS77-06-5級別BR別名赤霉酸；赤霉素A；2,4a,7-三羥基-1-甲基-8-亞甲基赤霉-3-烯-1,10-二羧酸-1,4a-內(nèi)酯；九二零純度90.0%分子式C19H22O6分子量346.37外觀（性狀）白色粉末儲存條件RT有效期2年溶解性50mg/ml EtOH規(guī)格5g單位瓶赤霉素是植物生長激素，能促進(jìn)莖、葉的生長，提早抽苔開花，促進(jìn)種子、塊莖、塊根發(fā)芽，刺激果實生長,增加結(jié)果率或形成無籽果實等.結(jié)馬鈴薯、蕃茄、稻、麥、棉花、大豆、豌豆、煙草、果樹等均有增產(chǎn)作用。但施用過多的赤霉素，植物會出現(xiàn)黃而細(xì)長的枝條，即失綠、徒長現(xiàn)象，反而影響產(chǎn)量。赤霉素還可用于從大麥制造麥芽。它對昆蟲的發(fā)育也有促進(jìn)作用。能溶于醇、酮、酯類等有機溶劑和pH值6.3的磷酸緩沖溶液，難溶于醚、氯仿、苯和水。與鉀、鈉離子形成鹽并溶于水。赤霉素固體比較穩(wěn)定，在干燥、密閉條件下貯存時間較久不會失效；水溶液不穩(wěn)定，低溫時短期貯存不致失效，溫度較高時分解較快；酸性或弱酸性條件較穩(wěn)定，堿性條件不穩(wěn)定。 備注：產(chǎn)品信息可能會有優(yōu)化升級。請以實際標(biāo)簽信息為準(zhǔn)。 備注：以上數(shù)據(jù)均來自公開文獻(xiàn), Solarbio暫未進(jìn)行獨立驗證, 僅供參考。These protocols are for reference only. Solarbio does not independently validate these methods.
TC處理細(xì)胞爬片48孔板用 直徑8mm
基本售價：420.00元
TC處理細(xì)胞爬片48孔板用 直徑8mm產(chǎn)品簡介規(guī)格100片/盒 100片/盒，50盒/箱單位盒細(xì)胞爬片是采用高端的玻片，經(jīng)滅菌，TC等處理后制作而成，厚度為0.17mm，用不同規(guī)格，適用于6孔，12孔，24孔，48孔的細(xì)胞培養(yǎng)板，拆開即用。TC玻片表面處理技術(shù)（TC處理→超強吸附），促進(jìn)細(xì)胞貼壁。即使在后期原位雜交，免疫組化、免疫熒光，激光共聚焦等處理過程中也不易脫片，避免傳統(tǒng)方法中從培養(yǎng)瓶轉(zhuǎn)移到載玻片上的損傷。一步法的細(xì)胞爬片不用預(yù)處理，避免在后期處理中細(xì)胞容易脫片等情況。?γ射線滅菌,無DNA酶、無RNA酶、無熱源。?爬片厚度為0.17mm,直徑為8mm/14mm/20mm/24mm.適用于48孔板/24孔板/12 孔板/6孔板? 雙面TC處理，雙面均可使用，避免了實驗誤差。? 爬片高強度耐酸堿，特殊實驗浸泡一個星期均不會碎片。? 一步法細(xì)胞爬片只需打開包裝即可使用，節(jié)省成本，節(jié)省實驗員的時間。使用說明：1.在超凈臺內(nèi)，使用75%乙醇擦拭外包裝鋁箔袋。2.拆開鋁箔袋，取出內(nèi)置方瓶后，用尖鑷將內(nèi)置爬片取出放入所用培養(yǎng)板或培養(yǎng)皿內(nèi)。3.種植細(xì)胞前，請首先用培養(yǎng)液或細(xì)胞懸液滴至爬片上，然后再加入培養(yǎng)液或細(xì)胞懸液，以避免爬片漂浮。操作注意事項:使用細(xì)胞爬片時(比如在六孔板中放入爬片，六孔板的孔底面積往往會比爬片面積多出一部分，加細(xì)胞懸液時常常就是細(xì)胞鋪滿整個孔底，有時細(xì)胞生長邊集現(xiàn)象，就是靠近壁邊緣密度要高些，這樣處于中央玻片上爬的細(xì)胞數(shù)量就可能相對較少) 比較好的做法是將玻片放入孔板的孔內(nèi)后，加細(xì)胞懸液時只滴到玻片上，一直滴到液體布滿整個玻片又不會溢出玻片邊緣(因為液體表面張力作用可以很容易做到這一點!)。放在培養(yǎng)箱里,等細(xì)胞貼壁后，取出，再滴加培養(yǎng)基布滿整個孔底,繼續(xù)培養(yǎng)，這樣玻片上的細(xì)胞生長的密度就會很集中。未用完的細(xì)胞爬片怎么保存?RT保存。細(xì)胞爬片有三層包裝，在操凈臺無菌的狀態(tài)下打開包裝，未用完的爬片按原先包裝方式再包裝起來即可。友情提示：該爬片表面帶有正電荷，請勿用手觸摸，以免使用效果不佳。玻片經(jīng)過γ射線滅菌后，透明玻片顏色會略變?yōu)榈枭?，屬正?，F(xiàn)象。備注：以上數(shù)據(jù)均來自公開文獻(xiàn), Solarbio暫未進(jìn)行獨立驗證, 僅供參考。These protocols are for reference only. Solarbio does not independently validate these methods.
維生素C
基本售價：240.00元
維生素C產(chǎn)品簡介CAS50-81-7中文名稱維生素C英文名稱L-Ascorbic Acid別名L(+)-Ascorbic acid;Vitamin C;L-抗壞血酸純度HPLC≥98%分子式C6H8O6 分子量176.12外觀（性狀）White to off-white Solid儲存條件Powder : 2-8℃, 2 years; In solvent(母液): -20℃, 1 month; -80℃, 6 months溶解性Soluble in Water ≥20mg/mLMDLMFCD00064328ECEINECS 200-066-2InChIKeyCIWBSHSKHKDKBQ-JLAZNSOCSA-NInChIInChI=1S/C6H8O6/c7-1-2(8)5-3(9)4(10)6(11)12-5/h2,5,7-10H,1H2/t2-,5+/m0/s1PubChem CID54670067SMILESOC([C@@]1([H])[C@H](CO)O)=C(O)C(O1)=O描述L-Ascorbic Acid是一種有效的還原劑和抗氧化劑。(L-Ascorbic Acid is a potent reducing agent and antioxidant.)生物活性L-Ascorbic acid exhibits anti-cancer effects through the generation of reactive oxygen species (ROS) and selective damage to cancer cells[1].In VitroThe anti-cancer effects of L-Ascorbic acid are determined by sodium-dependent vitamin C transporter 2 (SVCT-2), a transporter of L-ascorbic acid. L-Ascorbic acid (0.1 μM-2 mM) exhibits anti-cancer effects according to SVCT-2 expression and L-ascorbic acid uptake. Human colorectal cancer cell lines displays differential responses to L-ascorbic acid, primarily depending on the expression level of SVCT-2[1].In VivoL-Ascorbic acid/Tolbutamide produces hypoglycaemic activity in a dose dependant manner in normal (60 mg/kg) and diabetic (40 mg/kg) condition. In the presence of L-ascorbic acid, Tolbuatmide (20 mg/kg) produces early onset of action and maintained for longer period compared to Tolbutamide matching control[2].數(shù)據(jù)來源文獻(xiàn)[1]. Sungrae Cho, et al. Hormetic dose response to L-ascorbic acid as an anti-cancer drug in colorectal cancer cell lines according to SVCT-2 expression. Sci Rep. 2018 Jul 27;8(1):11372.[2]. Satyanarayana Sreemantula, et al. Influence of antioxidant (L- ascorbic acid) on tolbutamide induced hypoglycaemia/antihyperglycaemia in normal and diabetic rats. BMC Endocr Disord. 2005 Mar 3;5(1):2.規(guī)格20mg 10mM*1mL (in Water) 100mg單位瓶一種重要的營養(yǎng)素和氧化還原劑，在骨鹽代謝及骨形成中具有重要作用。在體外，它能增加鈣鹽的沉積，促進(jìn)礦化結(jié)節(jié)的形成，并通過間充質(zhì)細(xì)胞間接調(diào)節(jié)破骨細(xì)胞的分化。維生素C能直接抑制核因子κB受體活化子配體（RANKL）誘導(dǎo)的破骨細(xì)胞形成 。 使用本產(chǎn)品的應(yīng)用案例（僅供參考） In VitroCell（Osteogenic/成骨誘導(dǎo)；50 mg/l ascorbic acid） The osteogenic medium was the complete culture medium supplemented with 10 nM dexamethasone(Solarbio), 10 mM β-glycerophosphate (Solarbio), and 50 mg/l ascorbic acid (Solarbio). 數(shù)據(jù)來源文獻(xiàn)：Jia L, Zhang Y, Ji Y, Li X, Xing Y, Wen Y, Huang H, Xu X. Comparative analysis of lncRNA and mRNA expression profiles between periodontal ligament stem cells and gingival mesenchymal stem cells. Gene. 2019 May 30;699:155-164. doi: 10.1016/j.gene.2019.03.015. Epub 2019 Mar 12. PMID: 30876821. Cell（Osteogenic/成骨誘導(dǎo)；50 mg/l ascorbic acid） Osteogenic induced medium contained complete culture medium supplemented with 100 nM dexamethasone (Solarbio, Beijing, China), 10 mM β-glycerophosphate (Solarbio) and 50 mg/l ascorbic acid (Solarbio). The medium was replaced every other day. Alizarin Red–positive mineralized matrix was used to evaluate osteogenic capacity by Alizarin red (AR) staining. 數(shù)據(jù)來源文獻(xiàn)：Zhao Y, Liu H, Xi X, Chen S, Liu D. TRIM16 protects human periodontal ligament stem cells from oxidative stress-induced damage via activation of PICOT. Exp Cell Res. 2020 Dec 1;397(1):112336. doi: 10.1016/j.yexcr.2020.112336. Epub 2020 Oct 19. PMID: 33091421. In Vivo Rat（雄性新生SD Rat；100 mg vitamin C/kg bw/d；灌胃；30d） Forty-five male neonatal SD rats were randomly divided into five groups (n=9 each): corn oil control group, 500 mg DEHP/kg of body weight per day (bw/d) group, 500 mg DEHP/kg bw/d+200 mg vitamin E/kg bw/d group, 500 mg DEHP/kg bw/d+100 mg vitamin C/kg bw/d group, and 500 mg DEHP/kg bw/d+200 mg vitamin E/kg bw/d+100 mg vitamin C/kg bw/d group. DEHP and vitamin E (Solarbio, China) were dissolved in corn oil, and vitamin C (Solarbio,China) was dissolved in distilled water. From postnatal day (PND) 1 to 30, the male rats were administered corn oil, DEHP, vitamin C, and/or vitamin E by gavage each day according to their group. On PND 36, six rats of each groups were sacrificed and their testes were immediately isolated. The rest of rats were fed with routine feed until PND 90. 來源文獻(xiàn)：Tang X, Wu S, Shen L, Wei Y, Cao X, Wang Y, Long C, Zhou Y, Li D, Huang F, Liu B, Wei G. Di-(2-ethylhexyl) phthalate (DEHP)-induced testicular toxicity through Nrf2-mediated Notch1 signaling pathway in Sprague-Dawley rats. Environ Toxicol. 2018 Jul;33(7):720-728. doi: 10.1002/tox.22559. Epub 2018 Apr 16. PMID: 29663635. 備注：以上數(shù)據(jù)均來自公開文獻(xiàn), Solarbio暫未進(jìn)行獨立驗證, 僅供參考。These protocols are for reference only. Solarbio does not independently validate these methods
PerCP/Cyanine5.5 Anti-Human CD3抗體
基本售價：840.00元
PerCP/Cyanine5.5 Anti-Human CD3 Antibody[OKT-3]Uniprot : P07766 貨號: E-AB-F1001J 應(yīng)用: FCM Isotype: Mouse IgG2a, κ 宿主: Mouse Antigen Details Alternate NamesT-cell surface glycoprotein CD3 epsilon chain,CD3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3ESwissprotP07766 References1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.3. Lanier L, et al. 1986. J. Immunol. 137:2501.Product Details FormLiquidClone No.OKT-3 (See Other Available Formats) HostMouseIsotypeMouse IgG2a, κIsotype ControlPerCP/Cyanine5.5 Mouse IgG2a, κ Isotype Control[C1.18.4] [Product E-AB-F09802J] ReactivityHumanApplicationFCM (See the Application Description) Storage BufferPhosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.Recommended UseEach lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.ShippingBiological ice pack at 4℃Stability & StorageKeep as concentrated solution.Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.Centrifuge before opening to ensure complete recovery of vial contents.This product is guaranteed up to one year from purchase.ConjugationPerCP/Cyanine5.5PerCP/Cyanine5.5 is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).如何通過光譜圖選擇合適的檢測通道？ Background CD3ε is a 20 kD chain of the CD3/T cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
豬血清（無菌過濾）
基本售價：1100.00元
豬血清（無菌過濾）貨號：S9060 保存：-20°C 有效期：5 年 產(chǎn)品說明： 本產(chǎn)品采用豬血液為原料，無菌采集、批量混合，經(jīng) 0.1μm 微孔濾芯無菌過濾分裝而成。 本品未經(jīng)過滅活，溶液狀態(tài)下為黃色澄清液體，無溶血或異物，長時間靜置會有少量蛋白析出，使用前請震蕩混勻。 產(chǎn)品應(yīng)用（僅供參考）： 本品適合用于普通細(xì)胞培養(yǎng)，也可用于配制試劑用。 使用時需要注意以下幾點： 1. 血清一般儲存于-20℃，使用時為避免反復(fù)凍融，建議無菌環(huán)境下分裝成小包裝，儲存于-20℃。 2. 血清融化時最好先置于 2-8℃冰箱中使之溶解，然后在室溫下使之全溶，溶解過程中必須規(guī)則地?fù)u晃均勻。 3. 融化后的血清在 4℃不宜長時間存放，應(yīng)盡快使用 注意事項： 1. 產(chǎn)品信息僅供參考，如有疑問請致電 400-968-6088 咨詢。 2. 本產(chǎn)品僅供科研使用。請勿用于醫(yī)藥、臨床診斷或治療，食品及化妝品等用途。請勿存放于普通住宅區(qū)。 3. 為了您的安全和健康，請穿好實驗服并佩戴一次性手套和口罩操作。
轉(zhuǎn)鐵蛋白
基本售價：170.00元
轉(zhuǎn)鐵蛋白產(chǎn)品簡介英文名稱 Transferrin CAS 11096-37-0 純度 min.98% 外觀（性狀） 白色至淺黃色固體 儲存條件 2-8℃ 有效期 2年 溶解性 10 mg/mL in water 規(guī)格 10mg 單位 支 產(chǎn)品說明： 轉(zhuǎn)鐵蛋白又名運鐵蛋白 transferrin，TRF，siderophilin）是血漿中主要的含鐵蛋白質(zhì)，負(fù)責(zé)運載由消化管吸收的鐵和由紅細(xì)胞降解釋放的鐵。以 TRF-Fe3+的復(fù)合物形式進(jìn)入骨髓中，供成熟紅細(xì)胞的生成。 轉(zhuǎn)鐵蛋白是一種能傳遞鐵離子的蛋白質(zhì)。用于生化研究。 別名：鐵傳遞蛋白 鐵蛋白 人轉(zhuǎn)鐵蛋白 純度：98% 溶解性：10 mg/mL in water 注意事項： 1. 我司生產(chǎn)的生化試劑如無特殊標(biāo)注，基本為非無菌包裝，若用于細(xì)胞實驗，請?zhí)崆白龊妙A(yù)處理。 2. 一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。 3. 產(chǎn)品信息僅供參考，如有疑問請致電 400-968-6088 咨詢。 4. 本產(chǎn)品僅供科研使用。請勿用于醫(yī)藥、臨床診斷或治療，食品及化妝品等用途。請勿存放于普通住宅區(qū)。 5. 為了您的安全和健康，請穿好實驗服并佩戴一次性手套和口罩操作。
總抗氧化能力(T-AOC)檢測試劑盒（FRAP法）
基本售價：200.00元
總抗氧化能力(T-AOC)檢測試劑盒（FRAP法）產(chǎn)品簡介儲存條件2-8℃有效期6個月中文名稱總抗氧化能力(T-AOC)檢測試劑盒（FRAP法）英文名稱Total Antioxidant Capacity(T-AOC) Assay Kit（FRAP Method）檢測方法可見分光光度法可見分光光度法 Spectrophotometer單位盒規(guī)格50T/48S測定意義：測定對象中各種抗氧化物質(zhì)和抗氧化酶等構(gòu)成總抗氧化水平。在生物學(xué)、醫(yī)學(xué)和藥學(xué)研究中常常檢測血漿、血清、唾液、尿液等各種體液，細(xì)胞或組織等裂解液、植物或中草藥抽提液及各種抗氧化物(antioxidant)溶液的總抗氧化能力。 測定原理：在酸性環(huán)境下，物質(zhì)還原Fe3+-三吡啶三吖嗪(Fe3+-TPTZ)產(chǎn)生藍(lán)色的Fe2+-TPTZ的能力反映了其總抗氧化能力。 自備實驗用品：可見分光光度計、恒溫水浴鍋、低溫離心機、1mL玻璃比色皿和蒸餾水。 相關(guān)論文：A C2H2 zinc-finger protein OsZFP213 interacts with OsMAPK3 to enhance salt tolerance in rice - PubMed (nih.gov)
大鼠白介素11(IL-11)酶聯(lián)免疫吸附測定試劑盒
基本售價：3200.00元
Rat IL-11(Interleukin 11) ELISA Kit產(chǎn)品貨號:E-EL-R0562c 中文名:大鼠白介素11(IL-11)酶聯(lián)免疫吸附測定試劑盒別稱:IL11, AGIF, IL-11, interleukin 11用途 該試劑盒用于體外定量檢測Rat 血清，血漿或其他生物體液中IL-11濃度檢測原理 本試劑盒采用雙抗體夾心ELISA法。用抗大鼠IL-11抗體包被于酶標(biāo)板上，實驗時樣品或標(biāo)準(zhǔn)品中的大鼠IL-11會與包被抗體結(jié)合，游離的成分被洗去。依次加入生物素化的抗大鼠IL-11抗體和辣根過氧化物酶標(biāo)記的親和素?？勾笫驣L-11抗體與結(jié)合在包被抗體上的大鼠IL-11結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物，游離的成分被洗去。加入顯色底物(TMB)，TMB在辣根過氧化物酶的催化下呈現(xiàn)藍(lán)色，加終止液后變成黃色。用酶標(biāo)儀在450nm波長處測OD值，IL-11濃度與OD450值之間呈正比，通過繪制標(biāo)準(zhǔn)曲線計算出樣品中IL-11的濃度。反應(yīng)類型 夾心法規(guī)格 96T反應(yīng)時間 3.5h反應(yīng)性 Rat檢測方法 Colormetric檢測范圍 15.63—1000 pg/mL靈敏度 9.38 pg/mL樣本體積 100μL樣本類型 血清，血漿或其他生物體液特異性 可檢測樣本中的大鼠IL-11，且與其它相關(guān)蛋白無明顯交叉反應(yīng)。重復(fù)性 板內(nèi)，板間變異系數(shù)均<10%。典型數(shù)據(jù) 由于實驗操作條件的不同（如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等），標(biāo)準(zhǔn)曲線的OD值會有所差異。以下數(shù)據(jù)和曲線僅供參考，實驗者需根據(jù)自己的實驗建立標(biāo)準(zhǔn)曲線。(pg/mL) O.D Average Corrected 10002.4832.5052.4942.4195001.6431.6991.6711.5962500.990.9660.9780.9031250.450.4760.4630.38862.50.2740.2520.2630.18831.250.1820.170.1760.10115.630.1180.1360.1270.05200.0730.0770.075--精密度板內(nèi)精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 47.98127.48499.3948.54135.62511.36Standarddeviation 3.056.9720.282.997.5119.12C V (%) 6.365.474.066.165.543.74回收率 分別往5個不同樣本中添加已知濃度的目標(biāo)蛋白，做回收實驗，得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 94-109101EDTA plasma (n=8) 96-112102Cell culture media (n=8) 90-10195線性 分別往5個樣本中添加已知濃度的目標(biāo)蛋白，做回收實驗，得出回收率范圍及平均回收率。將5個樣本分別稀釋2倍，4倍，8倍，16倍做回收實驗，得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)93-10795-10996-110Average (%)981021031:4Range (%)91-10979-9285-97Average (%)9985901:8Range (%)89-10280-9387-100Average (%)9686921:16Range (%)93-10679-9387-99Average (%)1008694試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周；如果一周以后才使用試劑盒，請拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規(guī)格 保存條件 ELISA酶標(biāo)板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃，可存放6個月凍干標(biāo)準(zhǔn)品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結(jié)合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光)，可存放6個月標(biāo)準(zhǔn)品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結(jié)合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應(yīng)終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產(chǎn)品說明書Product Description1 份質(zhì)檢報告Certificate of Analysis1 份說明：所有試劑瓶蓋須旋緊以防止蒸發(fā)和微生物的污染。試劑體積以實際發(fā)貨版說明書為準(zhǔn)。相關(guān)試劑在分裝時會比標(biāo)簽上標(biāo)明的體積稍多一些，請在使用時量取而非直接倒出。試驗所需自備物品 1. 酶標(biāo)儀(450nm波長濾光片)2. 高精度移液器，EP管及一次性吸頭：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱，雙蒸水或去離子水4. 吸水紙操作步驟1. 對應(yīng)板孔中加入100μL標(biāo)準(zhǔn)品工作液或樣本，37℃孵育90分鐘2. 棄掉板內(nèi)液體后，立即加入100μL生物素化抗體工作液，37℃孵育60分鐘3. 棄掉板內(nèi)液體，洗板3次4. 每孔加入100μL HRP酶結(jié)合物工作液，37℃孵育30分鐘，棄掉板內(nèi)液體，洗板5次5. 每孔加入90μL底物溶液，37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長下讀數(shù)，處理數(shù)據(jù)
Recombinant human IL-6 protein, C-His
基本售價：3980.00元
Recombinant human IL-6 protein, C-His產(chǎn)品編號bs-41177P英文名稱Recombinant human IL-6 protein, C-His中文名稱重組人白介素6蛋白別 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6. ;Recombinant human IL-6 protein, C-His Specific References (1) | bs-41177P has been referenced in 1 publications.[IF=4.966] 222Xiaolong Zhang. et al. Baicalein inhibits non‐small‐cell lung cancer invasion and metastasis by reducing ezrin tension in inflammation microenvironment. Cancer Sci. 2020 Oct; 111(10): 3802–3812 other ; PubMed:32691974理論分子量15kDa檢測分子量21 kDa性 狀Lyophilized or Liquid濃 度＞0.5 mg/ml物 種Human序 列30-212/212純 度>95% as determined by SDS-PAGE純化方法AC內(nèi)毒素Not analyzed表達(dá)系統(tǒng)E.coli活性Not tested標(biāo)簽C-His緩 沖 液20 mM Tris-HCl (pH=8.0) with 150 mM NaCl保存條件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事項This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產(chǎn)品介紹Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. In humans, it is encoded by the IL6 gene. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response, e.g. during infection and after trauma, especially burns or other tissue damage leading to inflammation. IL-6 also plays a role in fighting infection, as IL-6 has been shown in mice to be required for resistance against bacterium Streptococcus pneumoniae.SWISS:P05231Gene ID:3569產(chǎn)品圖片
Recombinant mouse IL-6 protein, C-His
基本售價：1280.00元
Recombinant mouse IL-6 protein, C-His產(chǎn)品編號bs-10861P英文名稱Recombinant mouse IL-6 protein, C-His中文名稱重組小鼠白介素IL-6蛋白別 名IL6; Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; IL6_HUMAN; Interleukin6; Recombinant mouse IL-6 protein, C-His 理論分子量23kDa檢測分子量26 kDa性 狀Lyophilized or Liquid濃 度＞0.5 mg/ml物 種Mouse序 列25-211/211純 度>95% as determined by SDS-PAGE純化方法AC內(nèi)毒素Not analyzed表達(dá)系統(tǒng)E.coli活性Not tested標(biāo)簽C-His緩 沖 液20 mM Tris-HCl with 20% Glycerol保存條件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事項This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產(chǎn)品介紹This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq, Jun 2011].SWISS:P08505Gene ID:16193產(chǎn)品圖片
Recombinant human IL-22 protein
基本售價：4800.00元
Recombinant human IL-22 protein產(chǎn)品編號bs-43572P英文名稱Recombinant human IL-22 protein中文名稱重組人白介素22蛋白性 狀Lyophilized or Liquid濃 度1mg/ml保存條件Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.注意事項This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產(chǎn)品介紹產(chǎn)品圖片
Recombinant human IL-10 protein (Active, HEK293)
基本售價：2080.00元
Recombinant human IL-10 protein (Active, HEK293) 產(chǎn)品編號ck0006英文名稱Recombinant human IL-10 protein (Active, HEK293)中文名稱重組人白介素10 (活性的, Tag free)別 名Interleukin-10 precursor; Cytokine synthesis inhibitory factor; CSIF; Cytokine Synthesis Inhibitory Factor; IL 10; IL10A; Interleukin 10; Interleukin10; MGC126450; MGC126451; TGIF; IL10_HUMAN. ;Recombinant human IL-10 protein (Active, HEK293) 人白介素10 重組蛋白; 人白細(xì)胞介素10 ; 重組人白介素10; 重組人IL-10;理論分子量17kDa檢測分子量21 kDa性 狀Lyophilized powder濃 度＞0.5 mg/ml物 種Human序 列19-178/178純 度>95% as determined by SDS-PAGE純化方法AC內(nèi)毒素<0.5 EU/μg as determined by LAL.表達(dá)系統(tǒng)HEK293 cell活性Yes標(biāo)簽Tag free緩 沖 液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存條件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.注意事項This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.產(chǎn)品介紹Interleukin 10(IL-10) is a member of the IL-10 family of α-helical cytokines. IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of a number of proinflammatory cytokines such as IFNgamma, IL-2, IL-3, TNF and GM-CSF. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions.This recombinant human IL-10 is produced by human cells.(Tag free)Biological activity:The activity was measured by its ability to stimulate the proliferation of MC/9-2 cells (mouse master cell line).Reconstitution:Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin.SWISS:P18893Gene ID:3586產(chǎn)品圖片
Recombinant human IL-15 protein (Active, HEK293)
基本售價：2080.00元
Recombinant human IL-15 protein (Active, HEK293) 產(chǎn)品編號ck0013產(chǎn)品分類重組蛋白英文名稱Recombinant human IL-15 protein (Active, HEK293)中文名稱重組人白介素15 (活性的, Tag free)英文別名rhIL-15; Interleukin-15; IL 15; Interleukin 15; Interleukin15; MGC9721; IL15; IL15_HUMAN. ;Recombinant human IL-15 protein (Active, HEK293)中文別名人白介素15重組蛋白; 重組人白介素15; 人重組白介素15;純化方法AC檢測分子量14-15 kDaGeneID3600SwissP40933濃度＞0.5 mg/ml儲存液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存條件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.SubunitSubcellular LocationIsoform IL15-S48AA: Secreted. Isoform IL15-S21AA: Cytoplasm. Nucleus. Note=IL15-S21AA is not secreted, but rather is stored intracellularly, appearing in the nucleus and cytoplasmic components.Tissue SpecificityMost abundant in placenta and skeletal muscle. It is also detected in the heart, lung, liver and kidney. IL15-S21AA is preferentially expressed in tissues such as testis and thymus.SimilarityBelongs to the IL-15/IL-21 family.Database linksEntrez Gene: 3600 HumanOmim: 600554 HumanSwissProt: P40933 HumanUnigene: 654378 Human多肽活性Yes蛋白質(zhì)與多肽-多肽序列49-162/162小分子偶聯(lián)蛋白-蛋白載體標(biāo)簽Tag free純度>95% as determined by SDS-PAGE內(nèi)毒素<0.5 EU/μg as determined by LAL.重組蛋白-表達(dá)系統(tǒng)HEK293 cell復(fù)溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Recombinant human IL-2 protein (Active, HEK293)
基本售價：1080.00元
產(chǎn)品編號ck0017產(chǎn)品分類重組蛋白英文名稱Recombinant human IL-2 protein (Active, HEK293)中文名稱重組人白介素2 (活性的, Tag free)英文別名Interleukin-2; Interleukin 2; Interleukin2; rhIL-2; T-cell growth factor; T Cell Growth Factor; Aldesleukin; IL 2; IL2; Lymphokine; POIL2; T Cell Growth Factor; TCGF; Involved in regulation of T cell clonal expansion; IL2_HUMAN. ;Recombinant human IL-2 protein (Active, HEK293)中文別名人白介素2重組蛋白; 重組人白介素2; 人重組白介素2; 重組人白介素2（IL-2）;蛋白細(xì)胞定位分泌型蛋白純化方法AC理論分子量15kDa檢測分子量17 kDaGeneID3558SwissP60568濃度＞0.5 mg/ml儲存液Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.保存條件Use a manual defrost freezer and avoid repeated freeze-thaw cycles. In general: 12 months from date of receipt, -20 to -80℃ as supplied. 1 month, 2 to 8℃ under sterile conditions after reconstitution. 3 months, -20 to -80℃ under sterile conditions after reconstitution.SubunitSubcellular LocationSecreted.DISEASENote=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17.SimilarityBelongs to the IL-2 family.Database linksEntrez Gene: 3558 HumanEntrez Gene: 16183 MouseEntrez Gene: 116562 RatOmim: 147680 HumanSwissProt: P60568 HumanSwissProt: P04351 MouseSwissProt: P17108 RatUnigene: 89679 HumanUnigene: 14190 MouseUnigene: 9871 Rat背景資料The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]多肽活性Yes蛋白質(zhì)與多肽-多肽序列21-153/153小分子偶聯(lián)蛋白-蛋白載體標(biāo)簽Tag free純度>95% as determined by SDS-PAGE內(nèi)毒素<0.5 EU/μg as determined by LAL.重組蛋白-表達(dá)系統(tǒng)HEK293 cell復(fù)溶Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
人白介素15(IL-15)酶聯(lián)免疫吸附測定試劑盒
基本售價：3200.00元
Human IL-15(Interleukin 15) ELISA Kit文獻(xiàn)(2) 產(chǎn)品貨號:E-EL-H0222c 中文名:人白介素15(IL-15)酶聯(lián)免疫吸附測定試劑盒別稱:IL15用途 該試劑盒用于體外定量檢測Human 血清，血漿或其他生物體液中IL-15濃度檢測原理 本試劑盒采用雙抗體夾心ELISA法。用抗人IL-15抗體包被于酶標(biāo)板上，實驗時樣品或標(biāo)準(zhǔn)品中的人IL-15會與包被抗體結(jié)合，游離的成分被洗去。依次加入生物素化的抗人IL-15抗體和辣根過氧化物酶標(biāo)記的親和素?？谷薎L-15抗體與結(jié)合在包被抗體上的人IL-15結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物，游離的成分被洗去。加入顯色底物(TMB)，TMB在辣根過氧化物酶的催化下呈現(xiàn)藍(lán)色，加終止液后變成黃色。用酶標(biāo)儀在450nm波長處測OD值，IL-15濃度與OD450值之間呈正比，通過繪制標(biāo)準(zhǔn)曲線計算出樣品中IL-15的濃度。反應(yīng)類型 夾心法規(guī)格 96T反應(yīng)時間 3.5h反應(yīng)性 Human檢測方法 Colormetric檢測范圍 15.63—1000 pg/mL靈敏度 9.38 pg/mL樣本體積 100μL樣本類型 血清，血漿或其他生物體液特異性 可檢測樣本中的人IL-15，且與其它相關(guān)蛋白無明顯交叉反應(yīng)。重復(fù)性 板內(nèi)，板間變異系數(shù)均<10%。典型數(shù)據(jù) 由于實驗操作條件的不同（如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等），標(biāo)準(zhǔn)曲線的OD值會有所差異。以下數(shù)據(jù)和曲線僅供參考，實驗者需根據(jù)自己的實驗建立標(biāo)準(zhǔn)曲線。(pg/mL) O.D Average Corrected 10002.4852.5412.5132.4365001.6481.6681.6581.5812500.9160.8840.90.8231250.480.50.490.41362.50.2910.2710.2810.20431.250.1790.1770.1780.10115.630.1240.1340.1290.05200.0700.0840.077--精密度板內(nèi)精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 47.35146.58348.0446.47145.32338.28Standarddeviation 3.028.2715.043.166.4718.37C V (%) 6.385.644.326.804.455.43回收率 分別往5個不同樣本中添加已知濃度的目標(biāo)蛋白，做回收實驗，得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 92-10698EDTA plasma (n=8) 92-10397Cell culture media (n=8) 88-10394線性 分別往5個樣本中添加已知濃度的目標(biāo)蛋白，做回收實驗，得出回收率范圍及平均回收率。將5個樣本分別稀釋2倍，4倍，8倍，16倍做回收實驗，得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)96-11096-10984-97Average (%)101102901:4Range (%)88-10183-9584-96Average (%)9588911:8Range (%)91-10581-9289-99Average (%)9987941:16Range (%)93-10488-10088-100Average (%)999394試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周；如果一周以后才使用試劑盒，請拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規(guī)格 保存條件 ELISA酶標(biāo)板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃，可存放6個月凍干標(biāo)準(zhǔn)品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結(jié)合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光)，可存放6個月標(biāo)準(zhǔn)品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結(jié)合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應(yīng)終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產(chǎn)品說明書Product Description1 份質(zhì)檢報告Certificate of Analysis1 份說明：所有試劑瓶蓋須旋緊以防止蒸發(fā)和微生物的污染。試劑體積以實際發(fā)貨版說明書為準(zhǔn)。相關(guān)試劑在分裝時會比標(biāo)簽上標(biāo)明的體積稍多一些，請在使用時量取而非直接倒出。試驗所需自備物品 1. 酶標(biāo)儀(450nm波長濾光片)2. 高精度移液器，EP管及一次性吸頭：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱，雙蒸水或去離子水4. 吸水紙操作步驟1. 對應(yīng)板孔中加入100μL標(biāo)準(zhǔn)品工作液或樣本，37℃孵育90分鐘2. 棄掉板內(nèi)液體后，立即加入100μL生物素化抗體工作液，37℃孵育60分鐘3. 棄掉板內(nèi)液體，洗板3次4. 每孔加入100μL HRP酶結(jié)合物工作液，37℃孵育30分鐘，棄掉板內(nèi)液體，洗板5次5. 每孔加入90μL底物溶液，37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長下讀數(shù)，處理數(shù)據(jù)
人白介素23(IL-23)酶聯(lián)免疫吸附測定試劑盒
基本售價：3200.00元
Human IL-23(Interleukin 23) ELISA Kit文獻(xiàn)(10) 產(chǎn)品貨號:E-EL-H0107c 中文名:人白介素23(IL-23)酶聯(lián)免疫吸附測定試劑盒別稱:IL-23A, IL23P19, P19, SGRF用途 該試劑盒用于體外定量檢測Human 血清，血漿或其他生物體液中IL-23濃度檢測原理 本試劑盒采用雙抗體夾心ELISA法。用抗人IL-23抗體包被于酶標(biāo)板上，實驗時樣品或標(biāo)準(zhǔn)品中的人IL-23會與包被抗體結(jié)合，游離的成分被洗去。依次加入生物素化的抗人IL-23抗體和辣根過氧化物酶標(biāo)記的親和素。抗人IL-23抗體與結(jié)合在包被抗體上的人IL-23結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物，游離的成分被洗去。加入顯色底物(TMB)，TMB在辣根過氧化物酶的催化下呈現(xiàn)藍(lán)色，加終止液后變成黃色。用酶標(biāo)儀在450nm波長處測OD值，IL-23濃度與OD450值之間呈正比，通過繪制標(biāo)準(zhǔn)曲線計算出樣品中IL-23的濃度。反應(yīng)類型 夾心法規(guī)格 96T反應(yīng)時間 3.5h反應(yīng)性 Human檢測方法 Colormetric檢測范圍 39.06—2500 pg/mL靈敏度 23.44 pg/mL樣本體積 100μL樣本類型 血清，血漿或其他生物體液特異性 可檢測樣本中的人IL-23，且與其它相關(guān)蛋白無明顯交叉反應(yīng)。重復(fù)性 板內(nèi)，板間變異系數(shù)均<10%。典型數(shù)據(jù) 由于實驗操作條件的不同（如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等），標(biāo)準(zhǔn)曲線的OD值會有所差異。以下數(shù)據(jù)和曲線僅供參考，實驗者需根據(jù)自己的實驗建立標(biāo)準(zhǔn)曲線。(pg/mL) O.D Average Corrected 25002.1472.1892.1682.11512501.4851.5191.5021.4496250.830.7920.8110.758312.50.3640.3740.3690.316156.250.230.2020.2160.16378.130.1480.1320.140.08539.060.0930.1050.0990.04600.0450.0610.053--精密度板內(nèi)精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 136.57343.261072.26129.52356.171144.30Standarddeviation 7.2015.5535.498.4820.0254.47C V (%) 5.274.533.316.555.624.76回收率 分別往5個不同樣本中添加已知濃度的目標(biāo)蛋白，做回收實驗，得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 88-10094EDTA plasma (n=8) 94-10599Cell culture media (n=8) 87-10093線性 分別往5個樣本中添加已知濃度的目標(biāo)蛋白，做回收實驗，得出回收率范圍及平均回收率。將5個樣本分別稀釋2倍，4倍，8倍，16倍做回收實驗，得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)92-10795-10985-99Average (%)99102911:4Range (%)93-10484-9686-102Average (%)9890931:8Range (%)91-10683-9487-99Average (%)9989941:16Range (%)88-10384-9480-92Average (%)958987試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周；如果一周以后才使用試劑盒，請拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規(guī)格 保存條件 ELISA酶標(biāo)板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃，可存放6個月凍干標(biāo)準(zhǔn)品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結(jié)合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光)，可存放6個月標(biāo)準(zhǔn)品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結(jié)合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應(yīng)終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產(chǎn)品說明書Product Description1 份質(zhì)檢報告Certificate of Analysis1 份說明：所有試劑瓶蓋須旋緊以防止蒸發(fā)和微生物的污染。試劑體積以實際發(fā)貨版說明書為準(zhǔn)。相關(guān)試劑在分裝時會比標(biāo)簽上標(biāo)明的體積稍多一些，請在使用時量取而非直接倒出。試驗所需自備物品 1. 酶標(biāo)儀(450nm波長濾光片)2. 高精度移液器，EP管及一次性吸頭：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱，雙蒸水或去離子水4. 吸水紙操作步驟1. 對應(yīng)板孔中加入100μL標(biāo)準(zhǔn)品工作液或樣本，37℃孵育90分鐘2. 棄掉板內(nèi)液體后，立即加入100μL生物素化抗體工作液，37℃孵育60分鐘3. 棄掉板內(nèi)液體，洗板3次4. 每孔加入100μL HRP酶結(jié)合物工作液，37℃孵育30分鐘，棄掉板內(nèi)液體，洗板5次5. 每孔加入90μL底物溶液，37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長下讀數(shù)，處理數(shù)據(jù)
人可溶性白介素6受體(sIL-6R)酶聯(lián)免疫吸附測定試劑盒
基本售價：3200.00元
Human sIL-6R(Soluble Interleukin 6 Receptor) ELISA Kit產(chǎn)品貨號:E-EL-H2518c 中文名:人可溶性白介素6受體(sIL-6R)酶聯(lián)免疫吸附測定試劑盒別稱:IL6R, CD126, IL-6R-1, IL-6RA, IL6Q, IL6RA, IL6RQ, gp80, Interleukin-6 receptor, interleukin 6 receptor用途 該試劑盒用于體外定量檢測Human 血清，血漿或其他生物體液中sIL-6R濃度檢測原理 本試劑盒采用雙抗體夾心ELISA法。用抗人sIL-6R抗體包被于酶標(biāo)板上，實驗時樣品或標(biāo)準(zhǔn)品中的人sIL-6R會與包被抗體結(jié)合，游離的成分被洗去。依次加入生物素化的抗人sIL-6R抗體和辣根過氧化物酶標(biāo)記的親和素?？谷藄IL-6R抗體與結(jié)合在包被抗體上的人sIL-6R結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物，游離的成分被洗去。加入顯色底物(TMB)，TMB在辣根過氧化物酶的催化下呈現(xiàn)藍(lán)色，加終止液后變成黃色。用酶標(biāo)儀在450nm波長處測OD值，sIL-6R濃度與OD450值之間呈正比，通過繪制標(biāo)準(zhǔn)曲線計算出樣品中sIL-6R的濃度。反應(yīng)類型 夾心法規(guī)格 96T反應(yīng)時間 4.5h反應(yīng)性 Human檢測方法 Colormetric檢測范圍 15.63—1000 pg/mL靈敏度 9.38 pg/mL樣本體積 100μL樣本類型 血清，血漿或其他生物體液特異性 可檢測樣本中的人sIL-6R，且與其它相關(guān)蛋白無明顯交叉反應(yīng)。重復(fù)性 板內(nèi)，板間變異系數(shù)均<10%。典型數(shù)據(jù) 由于實驗操作條件的不同（如操作者、移液技術(shù)、洗板技術(shù)和穩(wěn)定條件等），標(biāo)準(zhǔn)曲線的OD值會有所差異。以下數(shù)據(jù)和曲線僅供參考，實驗者需根據(jù)自己的實驗建立標(biāo)準(zhǔn)曲線。(pg/mL) O.D Average Corrected 10002.4172.4692.4432.3845001.6951.7411.7181.6592500.9740.9390.9570.8981250.4350.4550.4450.38662.50.2680.2460.2570.19831.250.1750.1490.1620.10315.630.1060.1180.1120.05300.0490.0690.059--精密度板內(nèi)精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在1塊板子上檢測20次。板間精密度:低濃度樣本,中濃度樣本和高濃度樣本分別在3塊板子上檢測20次。Intra-assay Precision Inter-assay Precision Sample 123123n 202020202020Mean(pg/mL) 54.13124.28477.0256.05115.03491.14Standarddeviation 3.265.4422.943.755.2221.71C V (%) 6.024.384.816.694.544.42回收率 分別往5個不同樣本中添加已知濃度的目標(biāo)蛋白，做回收實驗，得出回收率范圍和平均回收率。Sample Type Range (%) Average Recovery (%) Serum (n=8) 83-9590EDTA plasma (n=8) 87-9792Cell culture media (n=8) 83-9590線性 分別往5個樣本中添加已知濃度的目標(biāo)蛋白，做回收實驗，得出回收率范圍及平均回收率。將5個樣本分別稀釋2倍，4倍，8倍，16倍做回收實驗，得出回收率范圍及平均回收率。Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5) 1:2Range (%)87-9998-11196-110Average (%)931051031:4Range (%)86-100101-11698-111Average (%)921071041:8Range (%)84-9797-10894-111Average (%)891021021:16Range (%)83-92100-11792-107Average (%)88108100試劑盒組成及保存 未拆封的試劑盒可在2-8℃保存一周；如果一周以后才使用試劑盒，請拆開試劑盒按照下表中的條件分別保存各組分。中文名稱 英文名稱 規(guī)格 保存條件 ELISA酶標(biāo)板(可拆卸)Micro ELISA Plate(Dismountable)8 孔×12 條-20℃，可存放6個月凍干標(biāo)準(zhǔn)品Reference Standard2 支濃縮生物素化抗體(100×)Concentrated Biotinylated Detection Ab1支 120 μL濃縮HRP酶結(jié)合物(100×)Concentrated HRP Conjugate1支 120 μL-20℃(避光)，可存放6個月標(biāo)準(zhǔn)品&樣品稀釋液Reference Standard & Sample Diluent1瓶 20 mL2-8℃.可存放6個月生物素化抗體稀釋液Biotinylated Detection Ab Diluent1瓶 14 mL酶結(jié)合物稀釋液HRP Conjugate Diluent1瓶 14 mL濃縮洗滌液(25×)Concentrated Wash Buffer (25×)1瓶 30 mL底物溶液(TMB)Substrate Reagent1瓶 10 mL2-8℃(避光)反應(yīng)終止液Stop Solution1瓶 10 mL2-8℃封板覆膜Plate Sealer5 張產(chǎn)品說明書Product Description1 份質(zhì)檢報告Certificate of Analysis1 份說明：所有試劑瓶蓋須旋緊以防止蒸發(fā)和微生物的污染。試劑體積以實際發(fā)貨版說明書為準(zhǔn)。相關(guān)試劑在分裝時會比標(biāo)簽上標(biāo)明的體積稍多一些，請在使用時量取而非直接倒出。試驗所需自備物品 1. 酶標(biāo)儀(450nm波長濾光片)2. 高精度移液器，EP管及一次性吸頭：0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL3. 37℃恒溫箱，雙蒸水或去離子水4. 吸水紙操作步驟1. 對應(yīng)板孔中加入100μL標(biāo)準(zhǔn)品工作液或樣本，37℃孵育90分鐘2. 棄掉板內(nèi)液體后，立即加入100μL生物素化抗體工作液，37℃孵育60分鐘3. 棄掉板內(nèi)液體，洗板3次4. 每孔加入100μL HRP酶結(jié)合物工作液，37℃孵育30分鐘，棄掉板內(nèi)液體，洗板5次5. 每孔加入90μL底物溶液，37℃孵育15分鐘左右6. 每孔加入50μL終止液7. 立即在450nm波長下讀數(shù)，處理數(shù)據(jù)
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